FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-KEPI/PPP1R14C antibody (AAA127688).Overlay histogram showing 293T cells stained with AAA127688 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEPI/PPP1R14C Antibody (AAA127688, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit KEPI/PPP1R14C Polyclonal Antibody | anti-KEPI/PPP1R14C antibody
Anti-KEPI/PPP1R14C Antibody Picoband
Gene Names
PPP1R14C; KEPI; NY-BR-81; CPI17-like
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
KEPI/PPP1R14C, Antibody; Anti-KEPI/PPP1R14C Antibody Picoband; interleukin 17B; Interleukin-17B; IL-17B; Cytokine CX1; Cytokine-like protein ZCYTO7; Neuronal interleukin-17-related factor; Il17b; Nirf, Zcyto7; anti-KEPI/PPP1R14C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-KEPI/PPP1R14C antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human KEPI/PPP1R14C recombinant protein (Position: H67-V165).
Subcellular Localization
Secreted
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-KEPI/PPP1R14C antibody (AAA127688).Overlay histogram showing 293T cells stained with AAA127688 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEPI/PPP1R14C Antibody (AAA127688, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-KEPI/PPP1R14C antibody (AAA127688).Overlay histogram showing 293T cells stained with AAA127688 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KEPI/PPP1R14C Antibody (AAA127688, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of KEPI/PPP1R14C using anti-KEPI/PPP1R14C antibody (AAA127688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: rat H9C2(2-1) whole cell lysates,Lane 4: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KEPI/PPP1R14C antigen affinity purified polyclonal antibody (#AAA127688) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KEPI/PPP1R14C at approximately 20 kDa. The expected band size for KEPI/PPP1R14C is at 20 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of KEPI/PPP1R14C using anti-KEPI/PPP1R14C antibody (AAA127688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: rat H9C2(2-1) whole cell lysates,Lane 4: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KEPI/PPP1R14C antigen affinity purified polyclonal antibody (#AAA127688) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KEPI/PPP1R14C at approximately 20 kDa. The expected band size for KEPI/PPP1R14C is at 20 kDa.)
Related Product Information for anti-KEPI/PPP1R14C antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-KEPI/PPP1R14C antibody
References
1. Liu, Q.-R., Zhang, P.-W., Zhen, Q., Walther, D., Wang, X.-B., Uhl, G. R. KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine. J. Biol. Chem. 277: 13312-13320, 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,843 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 14C
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory (inhibitor) subunit 14C
NCBI Official Symbol
PPP1R14C
NCBI Official Synonym Symbols
KEPI; NY-BR-81; CPI17-like
NCBI Protein Information
protein phosphatase 1 regulatory subunit 14C; kinase-enhanced PP1 inhibitor; kinase C-enhanced PP1 inhibitor; PKC-potentiated PP1 inhibitory protein; serologically defined breast cancer antigen NY-BR-81
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 14C
UniProt Gene Name
PPP1R14C
UniProt Synonym Gene Names
KEPI
UniProt Entry Name
PP14C_HUMAN
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Product Notes
The KEPI/PPP1R14C ppp1r14c (Catalog #AAA127688) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KEPI/PPP1R14C Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KEPI/PPP1R14C can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the KEPI/PPP1R14C ppp1r14c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KEPI/PPP1R14C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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