IHC (Immunohistochemisry)
(AAA322112 at 1/100 staining human colon cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit Keratin 16 Polyclonal Antibody | anti-KRT16 antibody
Keratin 16 Antibody
Gene Names
KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Keratin 16, Antibody; Keratin 16 Antibody; CK 16; CK-16; CK16; Cytokeratin-16; Cytokeratin16; FNEPPK; Focal non epidermolytic palmoplantar keratoderma; K 16; K16; K1C16_HUMAN; K1CP; Keratin 1 type I; Keratin 16; Keratin; Keratin type I cytoskeletal 16; Keratin-16; Keratin16; KRT 16; Krt16; KRT16A; NEPPK; PC1; type I cytoskeletal 16; anti-KRT16 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Keratin 16 antibody detects endogenous levels of total Keratin 16
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
473
Applicable Applications for anti-KRT16 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Keratin 16
Tissue Specificity
Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Predicted Cross Reactivity
Horse, Rabbit
Similarity
Horse (100%), Rabbit (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemisry)
(AAA322112 at 1/100 staining human colon cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry)
(AAA322112 at 1/100 staining human colon cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of Keratin 16 expression in HepG2 cell extract.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Keratin 16 expression in HepG2 cell extract.The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA322112 staining sw480 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA322112 staining sw480 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-KRT16 antibody
Description: Expressed in the hair follicle, nail bed and in mucosal stratified squamous epithelia and, suprabasally, in oral epithelium and palmoplantar epidermis. Also found in luminal cells of sweat and mammary gland ducts.
Function: Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
Subunit Structure: Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers (KRT6A or KRT6B) (By similarity). Interacts with TCHP (PubMed:15731013). Interacts with TRADD (By similarity).
Similarity: Belongs to the intermediate filament family.
Function: Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
Subunit Structure: Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers (KRT6A or KRT6B) (By similarity). Interacts with TCHP (PubMed:15731013). Interacts with TRADD (By similarity).
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 51 kDa
Predicted: 52 kDa
Predicted: 52 kDa
NCBI Official Full Name
keratin, type I cytoskeletal 16
NCBI Official Synonym Full Names
keratin 16
NCBI Official Symbol
KRT16
NCBI Official Synonym Symbols
K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
NCBI Protein Information
keratin, type I cytoskeletal 16
UniProt Protein Name
Keratin, type I cytoskeletal 16
UniProt Gene Name
KRT16
UniProt Synonym Gene Names
KRT16A; CK-16; K16
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Product Notes
The KRT16 krt16 (Catalog #AAA322112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Keratin 16 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 16 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT16 krt16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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