WB (Western Blot)
(Western blot analysis of extracts from HT29, using Phospho-Keratin 18 (Tyr36) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Rabbit Keratin 18 Polyclonal Antibody | anti-K18 antibody
Phospho-Keratin 18 (Tyr36) Antibody
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
Keratin 18, Antibody; Phospho-Keratin 18 (Tyr36) Antibody; Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D; keratin, type I cytoskeletal 18; Keratin-18; Krt18; anti-K18 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-Keratin 18 (Tyr36) Antibody detects endogenous levels of Keratin 18 only when phosphorylated at Tyr36.
Purity/Purification
Peptide affinity purification
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
430
Applicable Applications for anti-K18 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Keratin 18 around the phosphorylation site of Tyr36.
Preparation and Storage
Store at -20°C.
WB (Western Blot)
(Western blot analysis of extracts from HT29, using Phospho-Keratin 18 (Tyr36) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HT29, using Phospho-Keratin 18 (Tyr36) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of Phospho-Keratin 18 (Tyr36) in lysates of HT29, using Phospho-Keratin 18 (Tyr36) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-Keratin 18 (Tyr36) in lysates of HT29, using Phospho-Keratin 18 (Tyr36) Antibody.)
IHC (Immunohistochemistry)
((AAA328359) At 1/100 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
((AAA328359) At 1/100 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-K18 antibody
Description: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Post Translational Modifications: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Subunit Structure: Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Interacts with FAM83H (PubMed:23902688). Interacts with EPPK1 (By similarity).
Similarity: Belongs to the intermediate filament family.
Post Translational Modifications: Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Subunit Structure: Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Interacts with FAM83H (PubMed:23902688). Interacts with EPPK1 (By similarity).
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46 kDa
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
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Product Notes
The K18 krt18 (Catalog #AAA328359) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Keratin 18 (Tyr36) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 18 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the K18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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