WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells treated with Anisomycin, using Phospho-Keratin 20 (Ser13) Antibody.)
Rabbit anti-Human Keratin 20 Polyclonal Antibody | anti-KRT20 antibody
Phospho-Keratin 20 (Ser13) Antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
Keratin 20, Antibody; Phospho-Keratin 20 (Ser13) Antibody; CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20, type I; keratin 21, rat, homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20; anti-KRT20 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-Keratin 20 (Ser13) antibody detects endogenous levels of Keratin 20.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-KRT20 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Keratin 20 around the phosphorylation site of Ser13.
Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Predicted Cross Reactivity
Bovine, Sheep, Dog
Similarity
Bovine (100%), Sheep (83%), Dog (80%)
Conjugation
Unconjugated
Epitope
Phospho Ser13
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells treated with Anisomycin, using Phospho-Keratin 20 (Ser13) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells treated with Anisomycin, using Phospho-Keratin 20 (Ser13) Antibody.)
IHC (Immunohistochemistry)
(AAA321340 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321340 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-KRT20 antibody
Function: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Subunit Structure: Heterotetramer of two type I and two type II keratins. Associates with KRT8.
Post-translational Modifications: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Similarity: Belongs to the intermediate filament family.
Subunit Structure: Heterotetramer of two type I and two type II keratins. Associates with KRT8.
Post-translational Modifications: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kDa
Predicted: 49 kDa
Predicted: 49 kDa
NCBI Official Full Name
keratin, type I cytoskeletal 20
NCBI Official Synonym Full Names
keratin 20
NCBI Official Symbol
KRT20
NCBI Official Synonym Symbols
K20; CD20; CK20; CK-20; KRT21
NCBI Protein Information
keratin, type I cytoskeletal 20
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
Similar Products
Product Notes
The KRT20 krt20 (Catalog #AAA321340) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Keratin 20 (Ser13) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 20 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT20 krt20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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