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IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA229082 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Keratin, type I cytoskeletal 18 Polyclonal Antibody | anti-KRT18 antibody
Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Keratin, type I cytoskeletal 18, Antibody; Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody; Cell proliferation-inducing gene 46 protein Cytokeratin-18 Keratin-18 KRT18 CYK18 PIG46; anti-KRT18 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
430
Applicable Applications for anti-KRT18 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Keratin, type I cytoskeletal 18 chain protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-31701 / sc-31700 / sc-28264
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA229082 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA229082 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemistry)
(IHC image of AAA229082 diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image of AAA229082 diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate,Hela whole cell lysateAll lanes: KRT18 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49 KDaObserved band size: 49 KDa)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate,Hela whole cell lysateAll lanes: KRT18 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49 KDaObserved band size: 49 KDa)
WB (Western Blot)
(Western blot All lanes: KRT18 antibody at 12ug/mlLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48,66 kDa)
WB (Western Blot)
(Western blot All lanes: KRT18 antibody at 12ug/mlLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48,66 kDa)
WB (Western Blot)
(Western blotAll lanes: Keratin, type I cytoskeletal 18 antibody at 2ug/mlLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
WB (Western Blot)
(Western blotAll lanes: Keratin, type I cytoskeletal 18 antibody at 2ug/mlLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
Related Product Information for anti-KRT18 antibody
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells By similarity. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Product Categories/Family for anti-KRT18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18, type I
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
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Product Notes
The KRT18 krt18 (Catalog #AAA229082) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin, type I cytoskeletal 18 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin, type I cytoskeletal 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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