Rabbit Ki67/Mki67 Polyclonal Antibody | anti-Mki67 antibody

Anti-Ki67/Mki67 Antibody

Cat. Num. AAA125531

• Gene Names: Mki67; Ki67; Ki-67; D630048A14Rik
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Ki67/Mki67, Antibody; Anti-Ki67/Mki67 Antibody; Mki67; Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67 homolog; Antigen Ki67 homolog; antigen identified by monoclonal antibody Ki 67; anti-Mki67 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for Ki67/Mki67 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-Mki67 antibody: ELISA (Direct ELISA), IHC (Immunohistochemistry)
• Immunogen: E Coli-derived mouse Mki67 recombinant protein (Position: F29-S3177).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (AAA125531).Ki67/Mki67 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (AAA125531) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemisry)

(Figure 3. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (AAA125531).Ki67/Mki67 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (AAA125531) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry)

(Figure 2. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (AAA125531).Ki67/Mki67 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (AAA125531) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 1. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (AAA125531).Ki67/Mki67 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (AAA125531) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Related Product Information for anti-Mki67 antibody

Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

References

1. Fonatsch, C., Duchrow, M., Rieder, H., Schluter, C., Gerdes, J. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991.
2. Schonk, D. M., Kuijpers, H. J. H., vanDrunen, E., vanDalen, C. H., Geurts van Kessel, A. H. M., Verheijen, R., Ramaekers, F. C. S. Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum. Genet. 83: 297-299, 1989.
3. Traut, W., Scholzen, T., Winking, H., Kubbutat, M. H. G., Gerdes, J. Assignment of the murine Ki-67 gene (Mki67) to chromosome band 7F3-F5 by in situ hybridization. Cytogenet. Cell Genet. 83: 12-13, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 169234624
• NCBI GeneID: 17345
• NCBI Accession #: NP_001074586.2
• NCBI GenBank Nucleotide #: NM_001081117.2
• UniProt Accession #: E9PVX6; Q61769; Q7TSF6
• Molecular Weight: 324,718 Da
• NCBI Official Full Name: proliferation marker protein Ki-67
• NCBI Official Synonym Full Names: antigen identified by monoclonal antibody Ki 67
• NCBI Official Symbol: Mki67
• NCBI Official Synonym Symbols: Ki67; Ki-67; D630048A14Rik
• NCBI Protein Information: proliferation marker protein Ki-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: Mki67
• UniProt Synonym Gene Names: Antigen KI-67 homologCurated; Antigen Ki67 homologCurated