IF (Immunofluorescence)
(AAA328949 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328949 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit KIF17 Polyclonal Antibody | anti-KIF17 antibody
KIF17 Antibody
Gene Names
KIF17; KIF3X; KLP-2; OSM-3; KIF17B
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(88%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
KIF17, Antibody; KIF17 Antibody; KIAA1405; KIF 17; KIF 17B; KIF 3 related motor protein; KIF 3X; KIF17; KIF17_HUMAN; KIF17B; KIF3 related motor protein; KIF3-related motor protein; KIF3X; Kinesin family member 17; Kinesin like protein KIF 17; Kinesin like protein KIF17; Kinesin-like protein KIF17; anti-KIF17 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(88%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
KIF17 Antibody detects endogenous levels of total KIF17.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1028
Applicable Applications for anti-KIF17 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human KIF17, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328949 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328949 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328949 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328949 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohiostchemistry)
(AAA328949 at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328949 at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells, using KIF17 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 150 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from RAW264.7 cells, using KIF17 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 150 kDa.)
Related Product Information for anti-KIF17 antibody
Function: Transports vesicles containing N-methyl-D-aspartate (NMDA) receptor 2B along microtubules.
Subcellular Location: Cytoplasm>Cytoskeleton. Cell projection>Cilium.
Subunit Structure: Interacts with LIN-10 PDZ domain. Interacts with PIWIL1 (By similarity). Interacts with TBATA (By similarity).
Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Subcellular Location: Cytoplasm>Cytoskeleton. Cell projection>Cilium.
Subunit Structure: Interacts with LIN-10 PDZ domain. Interacts with PIWIL1 (By similarity). Interacts with TBATA (By similarity).
Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)115kD, 150kD.
Predicted Molecular Weight: (Calculated)115kDa.
Predicted Molecular Weight: (Calculated)115kDa.
NCBI Official Full Name
kinesin-like protein KIF17 isoform b
NCBI Official Synonym Full Names
kinesin family member 17
NCBI Official Symbol
KIF17
NCBI Official Synonym Symbols
KIF3X; KLP-2; OSM-3; KIF17B
NCBI Protein Information
kinesin-like protein KIF17
UniProt Protein Name
Kinesin-like protein KIF17
UniProt Gene Name
KIF17
UniProt Synonym Gene Names
KIAA1405; KIF3X
UniProt Entry Name
KIF17_HUMAN
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Product Notes
The KIF17 kif17 (Catalog #AAA328949) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIF17 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's KIF17 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the KIF17 kif17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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