WB (Western Blot)
(Western blot analysis of KIF23 expression in MKLP1 transfected 293T cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse KIF23 Polyclonal Antibody | anti-KIF23 antibody
KIF23 Antibody
Gene Names
KIF23; CHO1; KNSL5; MKLP1; MKLP-1
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KIF23, Antibody; KIF23 Antibody; CHO 1; CHO1; KIF 23; KIF23; KIF23_HUMAN; Kinesin family member 23; Kinesin like 5; Kinesin like protein 5; Kinesin like protein KIF 23; Kinesin like protein KIF23; Kinesin-like protein 5; Kinesin-like protein KIF23; KNS L5; KNSL 5; KNSL5; Mitotic kinesin like 1; Mitotic kinesin like protein 1; Mitotic kinesin-like protein 1; MKLP 1; anti-KIF23 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
KIF23 antibody detects endogenous levels of total KIF23
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
856
Applicable Applications for anti-KIF23 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Cytoplasm > Cytoskeleton > Spindle.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Similarity
Pig (85%), Bovine (85%), Horse (85%), Sheep (85%), Dog (85%), Chicken (83%), Xenopus (100%)
Conjugation
Unconjugated
WB (Western Blot)
(Western blot analysis of KIF23 expression in MKLP1 transfected 293T cell lysate, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of KIF23 expression in MKLP1 transfected 293T cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA323494 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323494 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-KIF23 antibody
Description: Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
Function: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
Subunit Structure: Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Interacts with ARF6, forming heterodimers and heterotetramers.
Post-translational Modifications: Ubiquitinated. Deubiquitinated by USP8/UBPY.
Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Function: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
Subunit Structure: Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY. Interacts with ARF6, forming heterodimers and heterotetramers.
Post-translational Modifications: Ubiquitinated. Deubiquitinated by USP8/UBPY.
Similarity: Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 89 kDa
Predicted: 111 kDa
Predicted: 111 kDa
NCBI Official Full Name
kinesin-like protein KIF23 isoform 2
NCBI Official Synonym Full Names
kinesin family member 23
NCBI Official Symbol
KIF23
NCBI Official Synonym Symbols
CHO1; KNSL5; MKLP1; MKLP-1
NCBI Protein Information
kinesin-like protein KIF23
UniProt Protein Name
Kinesin-like protein KIF23
UniProt Gene Name
KIF23
UniProt Synonym Gene Names
KNSL5; MKLP1
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Product Notes
The KIF23 kif23 (Catalog #AAA323494) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIF23 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIF23 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KIF23 kif23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF23, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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