Rabbit Kinesin 5A Polyclonal Antibody | anti-KIF5A antibody

Kinesin 5A Antibody

Cat. Num. AAA329037

• Gene Names: KIF5A; NKHC; ALS25; MY050; NEIMY; SPG10; D12S1889
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Kinesin 5A, Antibody; Kinesin 5A Antibody; D12S1889; KIF 5A; Kif5a; KIF5A_HUMAN; Kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin Heavy Chain Neuron Specific; Kinesin heavy chain neuron-specific 1; MY050; Neuronal kinesin heavy chain; NKHC 1; NKHC; SPG 10; anti-KIF5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Kinesin 5A Antibody detects endogenous levels of total Kinesin 5A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1032

• Applicable Applications for anti-KIF5A antibody: ELISA
• Immunogen: A synthesized peptide derived from human Kinesin 5A, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(AAA329037 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(AAA329037 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(AAA329037 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(AAA329037 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from various samples, using Kinesin 5A Antibody.Lane 1: K562, treated with blocking peptide;Lane 2: K562;Lane 3: 3T3.Observed bands: 160 kDa.)

Related Product Information for anti-KIF5A antibody

Function: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

Subcellular Location: Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Perikaryon. Note: Concentrated in the cell body of the neurons, particularly in the perinuclear region.

Tissue Specificity: Distributed throughout the CNS but is highly enriched in subsets of neurons.

Subunit Structure: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner. Interacts with ZFYVE27. Interacts with VAPA, VAPB, SURF4, RAB11A (GDP-bound form), RAB11B (GDP-bound form) and RTN3 in a ZFYVE27-dependent manner (By similarity). Interacts with BORCS5 (PubMed:25898167). Interacts with BICD2 (PubMed:20386726).

Similarity: Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 45446749
• NCBI GeneID: 3798
• NCBI Accession #: NP_004975.2
• NCBI GenBank Nucleotide #: NP_004975.2
• UniProt Accession #: Q12840
• Molecular Weight: Observed Molecular Weight: (Observed)117kD, 160kD.; Predicted Molecular Weight: (Calculated)117kDa.
• NCBI Official Full Name: kinesin heavy chain isoform 5A isoform 1
• NCBI Official Synonym Full Names: kinesin family member 5A
• NCBI Official Symbol: KIF5A
• NCBI Official Synonym Symbols: NKHC; ALS25; MY050; NEIMY; SPG10; D12S1889
• NCBI Protein Information: kinesin heavy chain isoform 5A
• UniProt Protein Name: Kinesin heavy chain isoform 5A
• UniProt Gene Name: KIF5A
• UniProt Synonym Gene Names: NKHC1; NKHC
• UniProt Entry Name: KIF5A_HUMAN