IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of Kir3.1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Rabbit Kir3.1 Polyclonal Antibody | anti-Kir3.1 antibody
Anti-Kir3.1 Antibody
Gene Names
KCNJ3; KGA; GIRK1; KIR3.1
Reactivity
Human, Mouse, Rat, Chicken, Bovine
Applications
Immunofluorescence, Western Blot
Synonyms
Kir3.1, Antibody; Anti-Kir3.1 Antibody; GIRK1; G protein-activated inward rectifier potassium channel 1; GIRK-1; Inward rectifier K (+) channel Kir3.1; Potassium channel; inwardly rectifying subfamily J member 3; anti-Kir3.1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Bovine
Clonality
Polyclonal
Applicable Applications for anti-Kir3.1 antibody
IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of Kir3.1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Dilution: IF WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Immunofluorescent analysis of Kir3.1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Western blot analysis of Kir3.1 expression in mouse brain (A) whole cell lysates.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Western blot analysis of Kir3.1 expression in mouse brain (A) whole cell lysates.)
Related Product Information for anti-Kir3.1 antibody
Rabbit polyclonal antibody to Kir3.1
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir3.1. The exact sequence is proprietary.
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir3.1. The exact sequence is proprietary.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,603 Da
NCBI Official Full Name
G protein-activated inward rectifier potassium channel 1 isoform 2
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 3
NCBI Official Symbol
KCNJ3
NCBI Official Synonym Symbols
KGA; GIRK1; KIR3.1
NCBI Protein Information
G protein-activated inward rectifier potassium channel 1; GIRK-1; inward rectifier K+ channel KIR3.1; inward rectifier K(+) channel Kir3.1; potassium channel, inwardly rectifying subfamily J member 3; potassium inwardly-rectifying channel subfamily J memb
UniProt Protein Name
G protein-activated inward rectifier potassium channel 1
UniProt Gene Name
KCNJ3
UniProt Synonym Gene Names
GIRK1; GIRK-1
UniProt Entry Name
IRK3_HUMAN
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Product Notes
The Kir3.1 kcnj3 (Catalog #AAA292824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kir3.1 Antibody reacts with Human, Mouse, Rat, Chicken, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Kir3.1 can be used in a range of immunoassay formats including, but not limited to, IC (Immunochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Kir3.1 kcnj3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kir3.1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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