Rabbit KISS1R Polyclonal Antibody | anti-KISS1R antibody

Phospho-KISS1R (Ser368) Antibody

Cat. Num. AAA328242

• Gene Names: KISS1R; HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: KISS1R, Antibody; Phospho-KISS1R (Ser368) Antibody; AXOR 12; AXOR12; G protein coupled receptor 54; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; GPCR 54; GPCR54; GPR 54; GPR54; hOT7T175; Hypogonadotropin 1; Hypogonadotropin-1; Hypogonadotropin1; KISS 1 receptor; KISS 1R; KiSS-1 receptor; KiSS-1R; KISS1 receptor; Kiss1r; Kisspeptins receptor; KISSR_HUMAN; Metastin receptor; OT7T175; anti-KISS1R antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-KISS1R(Ser368) Antibody detects endogenous levels of KISS1R only when phosphorylated at Ser368.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 398

• Applicable Applications for anti-KISS1R antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human KISS1R around the phosphorylation site of Ser368.
• Tissue Specificity: Most highly expressed in the pancreas, placenta and spinal cord, with lower-level of expression in peripheral blood leukocytes, kidney, lung, fetal liver, stomach, small intestine, testes, spleen, thymus, adrenal glands and lymph nodes. In the adult brain, expressed in the superior frontal gyrus, putamen, caudate nucleus, cingulate gyrus, nucleus accumbens, hippocampus, pons and amygdala, as well as the hypothalamus and pituitary. Expression levels are higher in early (7-9 weeks) than term placentas. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation. Also found in the extravillous trophoblast suggesting endocrine/paracrine activation mechanism.
• Subcellular Location: Plasma Membrane
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of Phospho-KISS1R(Ser368) in lysates of HepG2?, using Phospho-KISS1R(Ser368) Antibody.)

IHC (Immunohistochemisry)

(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohiostchemistry)

(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistochemistry)

(At 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Related Product Information for anti-KISS1R antibody

Description: Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.

Similarity: Belongs to the G-protein coupled receptor 1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 62865887
• NCBI GeneID: 84634
• NCBI Accession #: NP_115940.2
• NCBI GenBank Nucleotide #: NP_115940.2
• UniProt Accession #: Q969F8
• Molecular Weight: Observed: 43 kDa; Predicted: 43 kDa
• NCBI Official Full Name: kiSS-1 receptor
• NCBI Official Synonym Full Names: KISS1 receptor
• NCBI Official Symbol: KISS1R
• NCBI Official Synonym Symbols: HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
• NCBI Protein Information: kiSS-1 receptor
• UniProt Protein Name: KiSS-1 receptor
• UniProt Gene Name: KISS1R
• UniProt Synonym Gene Names: AXOR12; GPR54; KiSS-1R; hOT7T175
• UniProt Entry Name: KISSR_HUMAN