WB (Western Blot)
(Western blot analysis of KLDC2 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). KLDC2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse KLDC2 Polyclonal Antibody | anti-KLHDC2 antibody
KLDC2 Antibody (C-term)
Gene Names
KLHDC2; LCP; HCLP1; HCLP-1
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
KLDC2, Antibody; KLDC2 Antibody (C-term); Kelch domain-containing protein 2; Hepatocellular carcinoma-associated antigen 33; Host cell factor homolog LCP; Host cell factor-like protein 1; HCLP-1; KLHDC2; HCA33; anti-KLHDC2 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This KLDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-320 amino acids from the C-terminal region of human KLDC2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
293-320
Sequence Length
249
Applicable Applications for anti-KLHDC2 antibody
ELISA, WB (Western Blot)
Cellular Location
Nucleus
Tissue Location
Widely expressed, with high levels in skeletal muscle, heart, pancreas and liver (PubMed:11384994, PubMed:16964437) Undetectable in peripheral blood leukocytes (PubMed:16964437)
Tissue Location
Widely expressed, with high levels inskeletal muscle, heart, pancreas and liver.Undetectable in peripheral bloodleukocytes. EMBL; AF113131; AAD21038.1;-; mRNA EMBL; AF244137; AAF66246.1; -;mRNA EMBL; BX161429; CAD61901.1; -;mRNA EMBL; AK001771; BAA91898.1; -;mRNA EMBL; AK056298; BAG51671.1; -;mRNA EMBL; CR457205; CAG33486.1; -;mRNA EMBL; CH471078; EAW65749.1; -;Genomic_DNA EMBL; CH471078;EAW65750.1; -; Genomic_DNA EMBL;BC002335; AAH02335.1; -; mRNA EMBL;BC024192; AAH24192.1; -; mRNA CCDS;CCDS9693.1; -. [Q9Y2U9-1] RefSeq;NP_055130.1; NM_014315.2. [Q9Y2U9-1]PDB; 6DO3; X-ray; 2.17 A; A/B=1-362 PDB;6DO4; X-ray; 2.20 A; A/B=1-362 PDB;6DO5; X-ray; 2.50 A; A/B=1-362 PDBsum;6DO3; - PDBsum; 6DO4; - PDBsum; 6DO5; -SMR; Q9Y2U9; - BioGRID; 117123; 58IntAct; Q9Y2U9; 54 MINT; Q9Y2U9; -STRING; 9606.ENSP00000298307; -iPTMnet; Q9Y2U9; - PhosphoSitePlus;Q9Y2U9; - BioMuta; KLHDC2; - DMDM;28380093; - EPD; Q9Y2U9; - jPOST;Q9Y2U9; - MassIVE; Q9Y2U9; - PaxDb;Q9Y2U9; - PeptideAtlas; Q9Y2U9; - PRIDE;Q9Y2U9; - ProteomicsDB; 85905; -.[Q9Y2U9-1] ProteomicsDB; 85906; -.[Q9Y2U9-2] Antibodypedia; 23574; 93antibodies DNASU; 23588; - Ensembl;ENST00000298307; ENSP00000298307;ENSG00000165516. [Q9Y2U9-1] Ensembl;ENST00000555443; ENSP00000450944;ENSG00000165516. [Q9Y2U9-2] Ensembl;ENST00000555739; ENSP00000450539;ENSG00000165516. [Q9Y2U9-2] GeneID;23588; - KEGG; hsa:23588; - UCSC;uc001wwx.4; human. [Q9Y2U9-1] CTD;23588; - DisGeNET; 23588; - EuPathDB;HostDB:ENSG00000165516.10; -GeneCards; KLHDC2; - HGNC; HGNC:20231;KLHDC2 HPA; ENSG00000165516; Lowtissue specificity MIM; 611280; geneneXtProt; NX_Q9Y2U9; - OpenTargets;ENSG00000165516; - PharmGKB;PA134924955; - eggNOG; KOG0379;Eukaryota GeneTree;ENSGT00940000157150; - HOGENOM;CLU_1151487_0_0_1; - InParanoid; Q9Y2U9;- OMA; RYRDSRM; - OrthoDB;1263405at2759; - PhylomeDB; Q9Y2U9; -TreeFam; TF314081; - PathwayCommons;Q9Y2U9; - BioGRID-ORCS; 23588; 6 hits in876 CRISPR screens ChiTaRS; KLHDC2;human GenomeRNAi; 23588; - Pharos;Q9Y2U9; Tbio PRO; PR:Q9Y2U9; -Proteomes; UP000005640; Chromosome 14RNAct; Q9Y2U9; protein Bgee;ENSG00000165516; Expressed inquadriceps femoris and 246 other tissuesExpressionAtlas; Q9Y2U9; baseline anddifferential Genevisible; Q9Y2U9; HS GO;GO:0016604; C:nuclear body; IDA:HPA GO;GO:0031965; C:nuclear membrane;IDA:HPA GO; GO:0005654; C:nucleoplasm;IDA:HPA Gene3D; 2.120.10.80; -; 2 InterPro;IPR015915; Kelch-typ_b-propeller SUPFAM;SSF117281; SSF117281; 2 1: Evidence atprotein level; 3D-structure; Alternativesplicing; Kelch repeat; Nucleus; Referenceproteome; Repeat CHAIN 1..406/note="Kelch domain-containing protein 2"/id="PRO_0000119127" REPEAT 43..89/note="Kelch 1" REPEAT 102..155/note="Kelch 2" REPEAT 157..202/note="Kelch 3" REPEAT 229..278/note="Kelch 4" REPEAT 280..329/note="Kelch 5" REPEAT 331..377/note="Kelch 6" VAR_SEQ 239..249/note="DARMNDLHYLN -> VSIQTTSMTCF (inisoform 2)"/evidence="ECO:0000303|Ref.3"/id="VSP_030165" VAR_SEQ 250..406/note="Missing (in isoform 2)"/evidence="ECO:0000303|Ref.3"/id="VSP_030166" CONFLICT 25 /note="E-> D (in Ref. 5; CAG33486)"/evidence="ECO:0000305" STRAND 36..47/evidence="ECO:0000244|PDB:6DO3"STRAND 67..72/evidence="ECO:0000244|PDB:6DO3" TURN73..75/evidence="ECO:0000244|PDB:6DO3"STRAND 78..82/evidence="ECO:0000244|PDB:6DO3"STRAND 84..86/evidence="ECO:0000244|PDB:6DO3"STRAND 95..99/evidence="ECO:0000244|PDB:6DO3"STRAND 102..106/evidence="ECO:0000244|PDB:6DO3"STRAND 113..115/evidence="ECO:0000244|PDB:6DO3"STRAND 118..122/evidence="ECO:0000244|PDB:6DO3"STRAND 131..134/evidence="ECO:0000244|PDB:6DO3"STRAND 144..147/evidence="ECO:0000244|PDB:6DO3"STRAND 149..153/evidence="ECO:0000244|PDB:6DO3"STRAND 156..165/evidence="ECO:0000244|PDB:6DO3"STRAND 175..177/evidence="ECO:0000244|PDB:6DO3"HELIX 179..182/evidence="ECO:0000244|PDB:6DO3"STRAND 189..199/evidence="ECO:0000244|PDB:6DO3" TURN200..202/evidence="ECO:0000244|PDB:6DO3"STRAND 204..206/evidence="ECO:0000244|PDB:6DO3"STRAND 217..220/evidence="ECO:0000244|PDB:6DO5"STRAND 222..226/evidence="ECO:0000244|PDB:6DO3"STRAND 229..237/evidence="ECO:0000244|PDB:6DO3"STRAND 240..249/evidence="ECO:0000244|PDB:6DO3" TURN250..252/evidence="ECO:0000244|PDB:6DO3"STRAND 254..258/evidence="ECO:0000244|PDB:6DO3"STRAND 272..275/evidence="ECO:0000244|PDB:6DO3"STRAND 277..287/evidence="ECO:0000244|PDB:6DO3"STRAND 293..301/evidence="ECO:0000244|PDB:6DO3" TURN302..305/evidence="ECO:0000244|PDB:6DO3"STRAND 306..310/evidence="ECO:0000244|PDB:6DO3" TURN313..316/evidence="ECO:0000244|PDB:6DO4"STRAND 323..326/evidence="ECO:0000244|PDB:6DO3"STRAND 330..335/evidence="ECO:0000244|PDB:6DO3"STRAND 338..340/evidence="ECO:0000244|PDB:6DO3"HELIX 345..347/evidence="ECO:0000244|PDB:6DO3"STRAND 354..358/evidence="ECO:0000244|PDB:6DO3"SEQUENCE 406 AA; 46099 MW;07486B06AC4E97FF CRC64; MADGNEDLRADDLPGPAFES YESMELACPA ERSGHVAVSDGRHMFVWGGY KSNQVRGLYD FYLPREELWIYNMETGRWKK INTEGDVPPS MSGSCAVCVDRVLYLFGGHH SRGNTNKFYM LDSRSTDRVLQWERIDCQGI PPSSKDKLGV WVYKNKLIFFGGYGYLPEDK VLGTFEFDET SFWNSSHPRGWNDHVHILDT ETFTWSQPIT TGKAPSPRAAHACATVGNRG FVFGGRYRDA RMNDLHYLNLDTWEWNELIP QGICPVGRSW HSLTPVSSDHLFLFGGFTTD KQPLSDAWTY CISKNEWIQFNHPYTEKPRL WHTACASDEG EVIVFGGCANNLLVHHRAAH SNEILIFSVQ PKSLVRLSLEAVICFKEMLA NSWNCLPKHL LHSVNQRFGSNNTSGS
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of KLDC2 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). KLDC2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of KLDC2 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). KLDC2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of KLDC2 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). KLDC2 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of KLDC2 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). KLDC2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-KLHDC2 antibody
KLDC2 represses CREB3-mediated transcription by interfering with CREB3-DNA binding.
References
Chin, K.T., et al. Mol. Cell. Biochem. 296 (1-2), 109-119 (2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Wang, Y., et al. J. Immunol. 169(2):1102-1109(2002)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Wang, Y., et al. J. Immunol. 169(2):1102-1109(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
kelch domain-containing protein 2
NCBI Official Synonym Full Names
kelch domain containing 2
NCBI Official Symbol
KLHDC2
NCBI Official Synonym Symbols
LCP; HCLP1; HCLP-1
NCBI Protein Information
kelch domain-containing protein 2
UniProt Protein Name
Kelch domain-containing protein 2
UniProt Gene Name
KLHDC2
UniProt Synonym Gene Names
HCA33; HCLP-1
UniProt Entry Name
KLDC2_HUMAN
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Product Notes
The KLHDC2 klhdc2 (Catalog #AAA287562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 293-320. The KLDC2 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KLDC2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KLHDC2 klhdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLDC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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