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Application Data
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Application Data (Human lung cell line)

Rabbit KLF2 Polyclonal Antibody | anti-KLF2 antibody

KLF2 antibody - middle region

Gene Names
KLF2; LKLF
Reactivity
Human
Predicted: Guinea Pig, Human, Mouse, Pig, Rat, Zebrafish
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
KLF2; Polyclonal Antibody; KLF2 antibody - middle region; anti-KLF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted: Guinea Pig, Human, Mouse, Pig, Rat, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5mg/mL (varies by lot)
Sequence
Synthetic peptide located within the following region: PPAFGLFDDAAAAAAALGLAPPAARGLLTPPASPLELLEAKPKRGRRSWP
Sequence Length
355
Applicable Applications for anti-KLF2 antibody
Chromatin IP (ChIP), Immunohistochemistry (IHC), Western Blot (WB)
Homology
Guinea Pig: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rat: 93%; Zebrafish: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human KLF2
Protein Size
355 amino acids
Protein Interactions
KAT2B; EP300; CREBBP; FBXW7; UBC; GSK3B; ELAVL1; SMURF1; WWP1; GATA5; GATA6; WEE1
Additional Information
IHC Information: Brain, cerebellum
IHC Information: Colon, myenteric plexus
IHC Information: Lung
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Application Data

(Human lung cell line)

Application Data (Human lung cell line)

Application Data

(Human lung epithelial cell)

Application Data (Human lung epithelial cell)

WB (Western Blot)

(Host: RabbitTarget Name: KLF2Sample Type: Hela Whole cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/laneGel Concentration: 0.12%)

WB (Western Blot) (Host: RabbitTarget Name: KLF2Sample Type: Hela Whole cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/laneGel Concentration: 0.12%)

IHC (Immunohistchemistry)

(Immunohistochemistry with Human lung.)

IHC (Immunohistchemistry) (Immunohistochemistry with Human lung.)

IHC (Immunohistochemistry)

(Immunohistochemistry with Human Colon, myenteric plexus.)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Colon, myenteric plexus.)

WB (Western Blot)

(WB Suggested Anti-KLF2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate)

WB (Western Blot) (WB Suggested Anti-KLF2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate)

WB (Western Blot)

(Host: RabbitTarget Name: KLF2Sample Tissue: Human MCF7 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KLF2Sample Tissue: Human MCF7 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry)

(Immunohistochemistry with Human brain, cerebellum)

IHC (Immunohistochemistry) (Immunohistochemistry with Human brain, cerebellum)

ChIP (Chromatin Immunoprecipitation)

(Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)

ChIP (Chromatin Immunoprecipitation) (Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)
Related Product Information for anti-KLF2 antibody
This is a rabbit polyclonal antibody against KLF2. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: KLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.
Product Categories/Family for anti-KLF2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37kDa
NCBI Official Full Name
Krueppel-like factor 2
NCBI Official Synonym Full Names
Kruppel like factor 2
NCBI Official Symbol
KLF2
NCBI Official Synonym Symbols
LKLF
NCBI Protein Information
Krueppel-like factor 2
UniProt Protein Name
Krueppel-like factor 2
UniProt Gene Name
KLF2
UniProt Synonym Gene Names
LKLF

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Product Notes

The KLF2 klf2 (Catalog #AAA23416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLF2 antibody - middle region reacts with Human Predicted: Guinea Pig, Human, Mouse, Pig, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's KLF2 can be used in a range of immunoassay formats including, but not limited to, Chromatin IP (ChIP), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the KLF2 klf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: PPAFGLFDDA AAAAAALGLA PPAARGLLTP PASPLELLEA KPKRGRRSWP. It is sometimes possible for the material contained within the vial of "KLF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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