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product-image-AAA328245_WB13.jpg WB (Western Blot) (Western blot analysis of Phospho-KLF5(Ser303) in lysates of A549, using Phospho-KLF5(Ser303) Antibody.)

Rabbit KLF5 Polyclonal Antibody | anti-KLF5 antibody

Phospho-KLF5 (Ser303) Antibody

Gene Names
KLF5; CKLF; IKLF; BTEB2
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
KLF5, Antibody; Phospho-KLF5 (Ser303) Antibody; Basic transcription element binding protein 2; Basic transcription element-binding protein 2; BTE binding protein 2; BTE-binding protein 2; BTEB 2; BTEB2; CKLF; Colon krueppel like factor; Colon krueppel-like factor; GC box binding protein 2; GC-box-binding protein 2; IKLF; Intestinal enriched krueppel like factor; Intestinal-enriched krueppel-like factor; KLF 5; Klf5; KLF5_HUMAN; Krueppel like factor 5; Krueppel-like factor 5; Kruppel like factor 5 intestinal; Transcription factor BTEB 2; Transcription factor BTEB2; anti-KLF5 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-KLF5(Ser303) Antibody detects endogenous levels of KLF5 only when phosphorylated at Ser303.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
457
Applicable Applications for anti-KLF5 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human KLF5 around the phosphorylation site of Ser303.
Tissue Specificity
Expressed only in testis and placenta.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of Phospho-KLF5(Ser303) in lysates of A549, using Phospho-KLF5(Ser303) Antibody.)

product-image-AAA328245_WB13.jpg WB (Western Blot) (Western blot analysis of Phospho-KLF5(Ser303) in lysates of A549, using Phospho-KLF5(Ser303) Antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

product-image-AAA328245_IHC15.jpg IHC (Immunohistochemistry) (At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-KLF5 antibody
Description: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Post Translational Modifications: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Subunit Structure: Interacts with WWP1.

Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
688
UniProt Accession #
Molecular Weight
Observed: 51 kDa
Predicted: 51 kDa
NCBI Official Full Name
Krueppel-like factor 5
NCBI Official Synonym Full Names
Kruppel like factor 5
NCBI Official Symbol
KLF5
NCBI Official Synonym Symbols
CKLF; IKLF; BTEB2
NCBI Protein Information
Krueppel-like factor 5
UniProt Protein Name
Krueppel-like factor 5
UniProt Gene Name
KLF5
UniProt Synonym Gene Names
BTEB2; CKLF; IKLF; BTE-binding protein 2
UniProt Entry Name
KLF5_HUMAN

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Product Notes

The KLF5 klf5 (Catalog #AAA328245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-KLF5 (Ser303) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's KLF5 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KLF5 klf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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