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IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with KLF6 Antibody (C-term)(AAA286143).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human, mouse KLF6 Polyclonal Antibody | anti-KLF6 antibody
KLF6 Antibody (C-term)
Gene Names
KLF6; GBF; ZF9; BCD1; CBA1; CPBP; PAC1; ST12; COPEB
Reactivity
Human, mouse
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
KLF6, Antibody; KLF6 Antibody (C-term); Krueppel-like factor 6; B-cell-derived protein 1; Core promoter element-binding protein; GC-rich sites-binding factor GBF; Proto-oncogene BCD1; Suppressor of tumorigenicity 12 protein; Transcription factor Zf9; KLF6; BCD1; COPEB; CPBP; ST12; anti-KLF6 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This KLF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the C-terminal region of human KLF6.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
159-186
Sequence Length
237
Applicable Applications for anti-KLF6 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Function
Transcriptional activator (By similarity). Binds a GC box motif. Could play a role in B-cell growth and development.
Cellular Location
Nucleus
Tissue Location
Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with KLF6 Antibody (C-term)(AAA286143).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with KLF6 Antibody (C-term)(AAA286143).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF6 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF6 immunoreactivity is localized to Nucleus significantly.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using KLF6 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of widr cells using KLF6 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with KLF6 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of KLF6 Antibody (C-term) (AAA286143) in mouse stomach tissue lysates (35ug/lane). KLF6 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of KLF6 Antibody (C-term) (AAA286143) in mouse stomach tissue lysates (35ug/lane). KLF6 (arrow) was detected using the purified Pab.)
Related Product Information for anti-KLF6 antibody
COPEB is a member of the Kruppel-like family of transcription factors. The zinc finger protein is a transcriptional activator, and functions as a tumor suppressor.
Product Categories/Family for anti-KLF6 antibody
References
Sangodkar,J., Eur. J. Cancer 45 (4), 666-676 (2009) DiFeo,A., Drug Resist. Updat. 12 (1-2), 1-7 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Krueppel-like factor 6 isoform B
NCBI Official Synonym Full Names
Kruppel-like factor 6
NCBI Official Symbol
KLF6
NCBI Official Synonym Symbols
GBF; ZF9; BCD1; CBA1; CPBP; PAC1; ST12; COPEB
NCBI Protein Information
Krueppel-like factor 6
UniProt Protein Name
Krueppel-like factor 6
UniProt Gene Name
KLF6
UniProt Synonym Gene Names
BCD1; COPEB; CPBP; ST12
UniProt Entry Name
KLF6_HUMAN
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Product Notes
The KLF6 klf6 (Catalog #AAA286143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 159-186. The KLF6 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KLF6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KLF6 klf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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