Rabbit anti-Human, mouse KLHL6 Polyclonal Antibody | anti-KLHL6 antibody

KLHL6 Antibody (C-term)

Cat. Num. AAA288329

• Reactivity: Human, mouse
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: KLHL6, Antibody; KLHL6 Antibody (C-term); Kelch-like protein 6; KLHL6; anti-KLHL6 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This KLHL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-571 amino acids from the C-terminal region of human KLHL6.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-KLHL6 antibody: ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
• Antigen Region: 543-571
• Function: Involved in B-lymphocyte antigen receptor signaling and germinal center formation.
• Tissue Location: Found in germinal center B-cells.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(KLHL6 Antibody (C-term) (AAA288329) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(KLHL6 Antibody (C-term) (AAA288329) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLHL6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(KLHL6 Antibody (C-term) (AAA288329) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the KLHL6 antibody detected KLHL6 protein (arrow).)

WB (Western Blot)

(Anti-KLHL6 Antibody (C-term) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-KLHL6 antibody

Immunology

References

Gupta-Rossi, N., et al. Mol. Immunol. 39(13):791-799(2003)
Hattori, A., et al. DNA Res. 7(6):357-366(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 109150407
• NCBI GeneID: 89857
• NCBI Accession #: NP_569713.2
• NCBI GenBank Nucleotide #: NM_130446.2
• UniProt Accession #: Q8WZ60; Q8N5I1; Q8N892; B2RB31; D3DNS8
• Molecular Weight: 70359
• NCBI Official Full Name: kelch-like protein 6
• NCBI Official Synonym Full Names: kelch-like family member 6
• NCBI Official Symbol: KLHL6
• NCBI Protein Information: kelch-like protein 6
• UniProt Protein Name: Kelch-like protein 6
• UniProt Gene Name: KLHL6
• UniProt Entry Name: KLHL6_HUMAN