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WB (Western Blot)
(Western blot analysis of Phospho-KMT5A(Ser100) in lysates of 293T, using Phospho-KMT5A(Ser100) Antibody.)
Rabbit KMT5A Polyclonal Antibody | anti-KMT5A antibody
Phospho-KMT5A (Ser100) Antibody
Gene Names
KMT5A; SET8; SET07; SETD8; PR-Set7; PR/SET07
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
KMT5A, Antibody; Phospho-KMT5A (Ser100) Antibody; H4 K20 HMTase; H4 K20 specific histone methyltransferase; H4-K20-HMTase SETD8; Histone H4 K20 methyltransferase; Histone lysine N methyltransferase H4 lysine 20 specific; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; N-lysine methyltransferase SETD8; Pr SET 7; Pr SET7; PR-Set7; PR/SET domain containing protein 07; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PrSET7; SET 07; SET 8; SET domain containing (lysine methyltransferase) 8; SET domain containing 8; SET domain containing lysine methyltransferase 8; SET domain containing protein 8; SET domain-containing protein 8; SET07; SET8; SETD 8; setd8; SETD8_HUMAN; anti-KMT5A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-KMT5A(Ser100) Antibody detects endogenous levels of KMT5A only when phosphorylated at Ser100.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
393
Applicable Applications for anti-KMT5A antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human KMT5A around the phosphorylation site of Ser100.
Tissue Specificity
Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level).
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-KMT5A(Ser100) in lysates of 293T, using Phospho-KMT5A(Ser100) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-KMT5A(Ser100) in lysates of 293T, using Phospho-KMT5A(Ser100) Antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemisry)
(At 1/100 staining mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-KMT5A antibody
Description: Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Post Translational Modifications: Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity. Ubiquitinated and degraded by the DCX(DTL) complex.
Subunit Structure: Interacts with L3MBTL1. Isoform 2 interacts with SIRT2 (phosphorylated form); the interaction is direct, stimulates KMT5A-mediated methyltransferase activity at histone H4 'Lys-20' (H4K20me1) and is increased in a H2O2-induced oxidative stress-dependent manner.
Similarity: Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity. Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.
Post Translational Modifications: Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity. Ubiquitinated and degraded by the DCX(DTL) complex.
Subunit Structure: Interacts with L3MBTL1. Isoform 2 interacts with SIRT2 (phosphorylated form); the interaction is direct, stimulates KMT5A-mediated methyltransferase activity at histone H4 'Lys-20' (H4K20me1) and is increased in a H2O2-induced oxidative stress-dependent manner.
Similarity: Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity. Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 43 kDa
Predicted: 43 kDa
Predicted: 43 kDa
NCBI Official Full Name
N-lysine methyltransferase KMT5A
NCBI Official Synonym Full Names
lysine methyltransferase 5A
NCBI Official Symbol
KMT5A
NCBI Official Synonym Symbols
SET8; SET07; SETD8; PR-Set7; PR/SET07
NCBI Protein Information
N-lysine methyltransferase KMT5A
UniProt Protein Name
N-lysine methyltransferase SETD8
UniProt Gene Name
SETD8
UniProt Synonym Gene Names
KMT5A; PRSET7; SET07; SET8; PR-Set7; PR/SET07
UniProt Entry Name
SETD8_HUMAN
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Product Notes
The KMT5A setd8 (Catalog #AAA328254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-KMT5A (Ser100) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's KMT5A can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KMT5A setd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KMT5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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