WB (Western Blot)
(Detection of human KRAP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KRAP antibody AAA212805 (lot AAA212805-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human KRAP Polyclonal Antibody | anti-KRAP antibody
Rabbit anti-KRAP Antibody, Affinity Purified
Gene Names
SSFA2; CS1; CS-1; KRAP; SPAG13
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
KRAP, Antibody; Rabbit anti-KRAP Antibody, Affinity Purified; SPAG13; cleavage signal-1 protein; sperm-specific antigen 2; SSFA2; sperm specific antigen 2; sperm associated antigen 13; KRAS-induced actin-interacting protein; KRAP; ki-ras-induced actin-interacting protein; ITPR-interacting domain-containing protein 2; CS1; CS-1; Sperm-specific antigen 2; ITPR interacting domain containing 2; anti-KRAP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-KRAP antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1209 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human KRAP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KRAP antibody AAA212805 (lot AAA212805-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human KRAP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KRAP antibody AAA212805 (lot AAA212805-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human KRAP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KRAP antibody AAA212805 (lot AAA212805-1) used for IP at 6 ug per reaction. KRAP was also immunoprecipitated by rabbit anti-KRAP antibody BL17511. For blotting immunoprecipitated KRAP, AAA212805 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human KRAP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KRAP antibody AAA212805 (lot AAA212805-1) used for IP at 6 ug per reaction. KRAP was also immunoprecipitated by rabbit anti-KRAP antibody BL17511. For blotting immunoprecipitated KRAP, AAA212805 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-KRAP antibody
KRAS-induced actin-interacting protein (KRAP) is located near the plasma membrane. KRAP is associated with actin filaments and may also exist as a membrane-bound form with extracellular regions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P28290].
Product Categories/Family for anti-KRAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
138,386 Da
NCBI Official Full Name
sperm-specific antigen 2 isoform 1
NCBI Official Synonym Full Names
sperm specific antigen 2
NCBI Official Symbol
SSFA2
NCBI Official Synonym Symbols
CS1; CS-1; KRAP; SPAG13
NCBI Protein Information
sperm-specific antigen 2; cleavage signal-1 protein; sperm associated antigen 13; KRAS-induced actin-interacting protein; ki-ras-induced actin-interacting protein
UniProt Protein Name
Sperm-specific antigen 2
UniProt Gene Name
SSFA2
UniProt Synonym Gene Names
CS1; KIAA1927; KRAP; CS-1
UniProt Entry Name
SSFA2_HUMAN
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Product Notes
The KRAP ssfa2 (Catalog #AAA212805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KRAP Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRAP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KRAP ssfa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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