FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit KRT10 Polyclonal Antibody | anti-KRT10 antibody
KRT10 Antibody (N-term)
Gene Names
KRT10; BIE; EHK; K10; KPP; BCIE; CK10
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
KRT10, Antibody; KRT10 Antibody (N-term); Keratin; type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10; KRT10; KPP; anti-KRT10 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This KRT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human KRT10.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
150-179
Sequence Length
584
Applicable Applications for anti-KRT10 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of KRT10 Antibody (N-term) in 293 cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of KRT10 Antibody (N-term) in 293 cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)
Related Product Information for anti-KRT10 antibody
KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in its gene are associated with epidermolytic hyperkeratosis.
Product Categories/Family for anti-KRT10 antibody
References
Morais,P., Eur J Dermatol 19 (4), 333-336 (2009)
Barcelos,A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009)
Barcelos,A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58827
NCBI Official Full Name
keratin, type I cytoskeletal 10
NCBI Official Synonym Full Names
keratin 10, type I
NCBI Official Symbol
KRT10
NCBI Official Synonym Symbols
BIE; EHK; K10; KPP; BCIE; CK10
NCBI Protein Information
keratin, type I cytoskeletal 10
UniProt Protein Name
Keratin, type I cytoskeletal 10
UniProt Gene Name
KRT10
UniProt Synonym Gene Names
KPP; CK-10; K10
UniProt Entry Name
K1C10_HUMAN
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Product Notes
The KRT10 krt10 (Catalog #AAA289095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 150-179. The KRT10 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's KRT10 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KRT10 krt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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