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WB (Western Blot)
(Detection of human Lamin-A and Lamin-C by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A/C antibody AAA212163 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
Rabbit anti-Human, Mouse Lamin-A/C Polyclonal Antibody | anti-LMNA antibody
Rabbit anti-Lamin-A/C Antibody, Affinity Purified
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Lamin-A/C, Antibody; Rabbit anti-Lamin-A/C Antibody, Affinity Purified; prelamin-A/C; LDP1; LGMD1B; LMN1; LMNC; LMNL1; lamin; mandibuloacral dysplasia type A; lamin A/C-like 1; PRO1; renal carcinoma antigen NY-REN-32; MADA; CDCD1; LFP; 70 kDa lamin; IDC; CDDC; CMD1A; CMT2B1; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; EMD2; Prelamin-A/C; lamin A/C; anti-LMNA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-LMNA antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Pig
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Lamin-A and Lamin-C by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A/C antibody AAA212163 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
WB (Western Blot)
(Detection of human Lamin-A and Lamin-C by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A/C antibody AAA212163 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Lamin-A was also immunoprecipitated by rabbit anti-Lamin-A antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).)
IHC (Immunohistochemisry)
(Detection of mouse Lamin-A /C by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. AAA212163 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemisry)
(Detection of mouse Lamin-A /C by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. AAA212163 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Lamin-A /C by immunohistochemistry. Samples: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. AAA212163 Lot1) used at a dilution of 1:200 (1ug/ml) (left) and 1:1,000 (0.2ug/ml) (right). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Lamin-A /C by immunohistochemistry. Samples: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. AAA212163 Lot1) used at a dilution of 1:200 (1ug/ml) (left) and 1:1,000 (0.2ug/ml) (right). Detection: DAB)
ICC (Immunocytochemistry)
(Detection of human Lamin-A /C by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. AAA212163 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human Lamin-A /C by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Lamin-A/C (Cat. No. AAA212163 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
Related Product Information for anti-LMNA antibody
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin A/C plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P02545].
Product Categories/Family for anti-LMNA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,249 Da
NCBI Official Full Name
lamin isoform C
NCBI Official Synonym Full Names
lamin A/C
NCBI Official Symbol
LMNA
NCBI Official Synonym Symbols
FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
NCBI Protein Information
lamin; 70 kDa lamin; prelamin-A/C; lamin A/C-like 1; renal carcinoma antigen NY-REN-32
UniProt Protein Name
Prelamin-A/C
UniProt Gene Name
LMNA
UniProt Synonym Gene Names
LMN1
UniProt Entry Name
LMNA_HUMAN
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Product Notes
The LMNA lmna (Catalog #AAA212163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Lamin-A/C Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin-A/C can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LMNA lmna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin-A/C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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