IHC (Immunohistochemistry)
(Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated(bs-6713R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
Rabbit Laminin 5 Polyclonal Antibody | anti-LAMA3 antibody
Laminin 5 Polyclonal Antibody
Gene Names
LAMA3; E170; LOCS; BM600; LAMNA; lama3a
Reactivity
Rat, Pig, Dog, Bovine
Applications
Immunohistochemistry
Synonyms
Laminin 5, Antibody; Laminin 5 Polyclonal Antibody; Laminin subunit alpha-3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha; LAMA3; LAMNA; anti-LAMA3 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Bovine
Clonality
Polyclonal
Form/Format
0.01M TBS(pH 7.4), 0.09% (W/V) sodium azide and 50% Glycerol
Applicable Applications for anti-LAMA3 antibody
IHC (Immunohistochemistry)
Function
Binding to cells via a high affinity receptor , laminin is thougth to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane. Note: Major component
Tissue Location
Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS
Preparation and Storage
Store at -20 degree for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least 2 weeks at 2-4 degree.
IHC (Immunohistochemistry)
(Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated(bs-6713R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemistry)
(Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated(bs-6713R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 366,619
NCBI Official Full Name
laminin subunit alpha-3 isoform 2
NCBI Official Synonym Full Names
laminin, alpha 3
NCBI Official Symbol
LAMA3
NCBI Official Synonym Symbols
E170; LOCS; BM600; LAMNA; lama3a
NCBI Protein Information
laminin subunit alpha-3; BM600 150kD subunit; nicein 150kD subunit; nicein subunit alpha; kalinin 165kD subunit; kalinin subunit alpha; epiligrin subunit alpha; laminin-5 alpha 3 chain; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit a
UniProt Protein Name
Laminin subunit alpha-3
UniProt Gene Name
LAMA3
UniProt Synonym Gene Names
LAMNA; E170
UniProt Entry Name
LAMA3_HUMAN
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Product Notes
The LAMA3 lama3 (Catalog #AAA292051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Laminin 5 Polyclonal Antibody reacts with Rat, Pig, Dog, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin 5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the LAMA3 lama3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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