IHC (Immunohistochemistry)
(Immunohistochemical analysis of Laminin alpha 5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit anti-Human, Mouse Laminin alpha 5 Polyclonal Antibody | anti-LAMA5 antibody
Anti-Laminin alpha 5 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
Laminin alpha 5; Polyclonal Antibody; Anti-Laminin alpha 5 Antibody; KIAA0533; KIAA1907; Laminin subunit alpha-5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha; anti-LAMA5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Laminin alpha 5 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
3695
Applicable Applications for anti-LAMA5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1/500 - 1/1000
IHC: 1/100 - 1/200
IP: 1/10 - 1/100
IHC: 1/100 - 1/200
IP: 1/10 - 1/100
Entrez Gene
3911 (Human); 16776 (Mouse)
SwissProt
O15230 (Human); Q61001 (Mouse)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Laminin alpha 5. The exact sequence is proprietary.
Preparation and Storage
Upon receipt aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Laminin alpha 5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Laminin alpha 5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of Laminin alpha 5 expression in JAR (A), A431 (B), RAW264.7 (C) whole cell lysates.(Predicted band size: 399 kD; Observed band size: 400 kD))
WB (Western Blot)
(Western blot analysis of Laminin alpha 5 expression in JAR (A), A431 (B), RAW264.7 (C) whole cell lysates.(Predicted band size: 399 kD; Observed band size: 400 kD))
Related Product Information for anti-LAMA5 antibody
Rabbit polyclonal antibody to Laminin alpha 5
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
laminin subunit alpha-5
NCBI Official Synonym Full Names
laminin, alpha 5
NCBI Official Symbol
LAMA5
NCBI Protein Information
laminin subunit alpha-5; laminin alpha-5 chain; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha
UniProt Protein Name
Laminin subunit alpha-5
UniProt Gene Name
LAMA5
UniProt Synonym Gene Names
KIAA0533; KIAA1907
UniProt Entry Name
LAMA5_HUMAN
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Product Notes
The LAMA5 lama5 (Catalog #AAA17816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Laminin alpha 5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Laminin alpha 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1/500 - 1/1000 IHC: 1/100 - 1/200 IP: 1/10 - 1/100. Researchers should empirically determine the suitability of the LAMA5 lama5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Laminin alpha 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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