Rabbit anti-Human LAMP1 Polyclonal Antibody | anti-LAMP1 antibody

Anti-LAMP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA255358

• Gene Names: LAMP1; LAMPA; CD107a; LGP120
• Reactivity: Human
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: LAMP1, Antibody; Anti-LAMP1 Antibody, Rabbit Polyclonal; LAMP1/CD107a Antibody, Rabbit PAb, Antigen Affinity Purified; lysosomal-associated membrane protein 1; Anti-CD107a Antibody; Anti-LAMPA Antibody; Anti-LGP120 Antibody; anti-LAMP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LAMP1/CD107a
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LAMP1 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Human LAMP1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human LAMP1 extracellular domain (rh LAMP1). LAMP1 specific IgG was purified by human LAMP1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(LAMP1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell LysateLane D:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-LAMP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LAMP1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 113 kDa)

WB (Western Blot)

(Anti-LAMP1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:120 kDaObserved band size:130 kDa)

Related Product Information for anti-LAMP1 antibody

Lysosome-associated membrane glycoprotein 1, also known as CD107 antigen-like family member A, CD107a, and LAMP1, is a single-pass type I membrane protein that belongs to the LAMP family. CD107a is expressed largely in the endosome-lysosome membranes of cells but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD107b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in which amyloid burden was cleared following beta A-peptide immunization. Also, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide, or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.

References

Parkinson-Lawrence EJ, et al. (2005) Immunochemical analysis of CD107a (LAMP-1). Cell Immunol. 236(1-2): 161-6.Barrachina M, et al. (2006) Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 32(5): 505-16.Zhang H, et al. (2010) Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Arch Oral Biol. 55(6): 417-25.

NCBI and Uniprot Product Information

• NCBI GI #: 112380628
• NCBI GeneID: 3916
• NCBI Accession #: NP_005552.3
• NCBI GenBank Nucleotide #: NM_005561.3
• UniProt Accession #: P11279; Q8WU33; Q96I40; Q9BRD2; Q9NP13
• Molecular Weight: 44,882 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 1
• NCBI Official Symbol: LAMP1
• NCBI Official Synonym Symbols: LAMPA; CD107a; LGP120
• NCBI Protein Information: lysosome-associated membrane glycoprotein 1; LAMP-1; CD107 antigen-like family member A; lysosome-associated membrane protein 1
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 1
• UniProt Gene Name: LAMP1
• UniProt Synonym Gene Names: LAMP-1
• UniProt Entry Name: LAMP1_HUMAN