Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us
product-image-AAA212692_WB10.jpg WB (Western Blot) (Detection of human and mouse LAS1L by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-LAS1L antibody AAA212692 (lot AAA212692-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

Rabbit anti-Human, Mouse LAS1L Polyclonal Antibody | anti-LAS1L antibody

Rabbit anti-LAS1L Antibody, Affinity Purified

Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
LAS1L, Antibody; Rabbit anti-LAS1L Antibody, Affinity Purified; protein LAS1 homolog; ribosomal biogenesis protein LAS1L; Las1-like; dJ475B7.2; WTS; Ribosomal biogenesis protein LAS1L; LAS1 like, ribosome biogenesis factor; anti-LAS1L antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-LAS1L antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 684 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse LAS1L by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-LAS1L antibody AAA212692 (lot AAA212692-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212692_WB10.jpg WB (Western Blot) (Detection of human and mouse LAS1L by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-LAS1L antibody AAA212692 (lot AAA212692-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human LAS1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-LAS1L antibody AAA212692 (lot AAA212692-1) used for IP at 6 ug per reaction. LAS1L was also immunoprecipitated by rabbit anti-LAS1L antibody For blotting immunoprecipitated LAS1L, AAA212692 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

product-image-AAA212692_IP11.jpg IP (Immunoprecipitation) (Detection of human LAS1L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-LAS1L antibody AAA212692 (lot AAA212692-1) used for IP at 6 ug per reaction. LAS1L was also immunoprecipitated by rabbit anti-LAS1L antibody For blotting immunoprecipitated LAS1L, AAA212692 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohiostchemistry)

(Detection of human LAS1L by immunohistochemistry. Samples: FFPE serial sections of human testicular seminoma. Antibody: Affinity purified rabbit anti-LAS1L (Cat. No. Lot1, left image and Cat. No. AAA212692 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212692_IHC13.jpg IHC (Immunohiostchemistry) (Detection of human LAS1L by immunohistochemistry. Samples: FFPE serial sections of human testicular seminoma. Antibody: Affinity purified rabbit anti-LAS1L (Cat. No. Lot1, left image and Cat. No. AAA212692 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of mouse LAS1L by immunohistochemistry. Samples: FFPE serial sections of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-LAS1L (Cat. No. Lot1, left image and Cat. No. AAA212692 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212692_IHC15.jpg IHC (Immunohistochemistry) (Detection of mouse LAS1L by immunohistochemistry. Samples: FFPE serial sections of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-LAS1L (Cat. No. Lot1, left image and Cat. No. AAA212692 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-LAS1L antibody
LAS1L is involved in the biogenesis of the 60S ribosomal subunit. It is required for maturation of the 28S rRNA. LAS1L functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y4W2].
Product Categories/Family for anti-LAS1L antibody

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #
UniProt Protein Name
Ribosomal biogenesis protein LAS1L
UniProt Gene Name
LAS1L
UniProt Synonym Gene Names
MSTP060
UniProt Entry Name
LAS1L_HUMAN

Similar Products

Product Notes

The LAS1L las1l (Catalog #AAA212692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LAS1L Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LAS1L can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LAS1L las1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAS1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Submit a Question

Please complete the form below and a representative will contact you as soon as possible.

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.