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WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit LC3 Polyclonal Antibody | anti-MAP1LC3B antibody
LC3 Antibody (APG8B) (N-term)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Applications
Immunofluorescence, ELISA, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LC3, Antibody; LC3 Antibody (APG8B) (N-term); Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; anti-MAP1LC3B antibody
Host
Rabbit
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.1 (varies by lot)
Sequence Positions
1-30
Sequence Length
125
Applicable Applications for anti-MAP1LC3B antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of anti-LC3 (APG8b) in untreated or treated Hela cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).)
WB (Western Blot)
(Western blot analysis of anti-LC3 (APG8b) in untreated or treated Hela cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).)
WB (Western Blot)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B) (N-term). AAA28778 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B) (N-term). AAA28778 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B) (N-term). AAA28778 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from mouse NIH/3T3 cell line, using LC3 Antibody (APG8B) (N-term). AAA28778 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line, untreated or treated with chloroquine, 50uM, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
Related Product Information for anti-MAP1LC3B antibody
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Product Categories/Family for anti-MAP1LC3B antibody
References
References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14688
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3B
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol
MAP1LC3B
NCBI Official Synonym Symbols
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3B
UniProt Gene Name
MAP1LC3B
UniProt Synonym Gene Names
MAP1ALC3; MAP1A/MAP1B LC3 B
UniProt Entry Name
MLP3B_HUMAN
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Product Notes
The MAP1LC3B map1lc3b (Catalog #AAA28778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The LC3 Antibody (APG8B) (N-term) reacts with Human, rat (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's LC3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MAP1LC3B map1lc3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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