Rabbit LC3B Polyclonal Antibody | anti-LC3B antibody

LC3B Rabbit Polyclonal Antibody

Cat. Num. AAA30772

• Gene Names: MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: LC3B, Antibody; LC3B Rabbit Polyclonal Antibody; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; YM3381; BH6842; anti-LC3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous MAP LC3B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-LC3B antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-2000; IHC: 1:200-500; IF: 1:200
• Immunogen: Recombinant Protein of MAP LC3B
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

Application Data

(Jia, Wei?Qiang, et al. "Verbascoside inhibits progression of glioblastoma cells by promoting Let?7g?5p and down?regulating HMGA2 via Wnt/beta?catenin signalling blockade." Journal of cellular and molecular medicine 24.5 (2020): 2901-2916.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). LC3B Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).)

WB (Western Blot)

(Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Rat Brain Tissue cells, ?Green? primary antibody was diluted at 1:1000, 4 degree over night, secondary antibody was diluted at 1:10000, 37 degree 1hour. ?Red? GAPDH Monoclonal Antibody(2B8) antibody was diluted at 1:5000 as loading control, 4 degree over night,secondary antibody was diluted at 1:10000, 37 degree 1hour.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

WB (Western Blot)

(Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using MAP LC3? Polyclonal Antibody. Secondary antibody was diluted at 1:20000)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using MAP LC3? Polyclonal Antibody.)

Application Data

(Feng, Qin. "Quantitative proteomic analysis reveals that Arctigenin alleviates concanavalin A-induced hepatitis through suppressing immune system and regulating autophagy." Frontiers in immunology 9 (2018): 1881.)

Related Product Information for anti-LC3B antibody

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of Cytoplasmic component. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 17433141
• NCBI GeneID: 81631
• UniProt Accession #: Q9GZQ8; Q6NW02
• Molecular Weight: 125
• NCBI Official Full Name: Microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 beta
• NCBI Official Symbol: MAP1LC3B
• NCBI Official Synonym Symbols: LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• UniProt Gene Name: MAP1LC3B
• UniProt Synonym Gene Names: MAP1ALC3; MAP1A/MAP1B LC3 B
• UniProt Entry Name: MLP3B_HUMAN