IF (Immunofluorescence)
(AAA328863 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328863) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Rabbit anti-Human LC3C-Specific Polyclonal Antibody | anti-LC3C antibody
LC3C-Specific Antibody
Gene Names
MAP1LC3C; LC3C; ATG8J
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Synonyms
LC3C-Specific, Antibody; LC3C-Specific Antibody; ATG8J; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; LC3 like protein 2; MAP1 light chain 3 like protein 2; MAP1 light chain 3 like protein 3; MAP1 light chain 3-like protein 3; MAP1A/MAP1B LC3 C; MAP1A/MAP1B light chain 3 C; MAP1LC3C; Microtubule-associated protein 1 light chain 3 gamma; Microtubule-associated proteins 1A/1B light chain 3C; MLP3C_HUMAN; anti-LC3C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
LC3C Antibody detects endogenous levels of total LC3C.
Purity/Purification
Peptide affinity purification
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LC3C antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human LC3C,corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328863 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328863) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328863 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328863) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from Chloroquine treated HepG2 cells, using LC3C-Specific Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Chloroquine treated HepG2 cells, using LC3C-Specific Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-LC3C antibody
Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruits all ATG8 family members to infecting bacteria such as S.Typhimurium (PubMed:23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed:28404643).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17 kDa
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3C
NCBI Official Synonym Full Names
microtubule associated protein 1 light chain 3 gamma
NCBI Official Symbol
MAP1LC3C
NCBI Official Synonym Symbols
LC3C; ATG8J
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3C
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3C
UniProt Gene Name
MAP1LC3C
UniProt Synonym Gene Names
MAP1A/MAP1B LC3 C
UniProt Entry Name
MLP3C_HUMAN
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Product Notes
The LC3C map1lc3c (Catalog #AAA328863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LC3C-Specific Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LC3C-Specific can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the LC3C map1lc3c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LC3C-Specific, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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