WB (Western Blot)
(Western blot analysis of extracts of MOLT-15 cells treated with phosphatase, using Phospho-LCP1 (Tyr28) Antibody.)
Rabbit anti-Human, Mouse LCP1 Polyclonal Antibody | anti-LCP1 antibody
Phospho-LCP1 (Tyr28) Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
LCP1, Antibody; Phospho-LCP1 (Tyr28) Antibody; CP64; L plastin; L-plastin; Larval cuticle protein 1; LC64P; LCP-1; LCP1; LPL; Lplastin; Lymphocyte cytosolic protein 1; Plastin 2; Plastin-2; PLS2; PLSL_HUMAN; anti-LCP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-LCP1 (Tyr28) antibody detects endogenous levels of LCP1.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
627
Applicable Applications for anti-LCP1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human LCP1 around the phosphorylation site of Tyr28.
Subcellular Location
Cytoplasm > Cytoskeleton. Cell Junction. Cell Projection. Cell Projection > Ruffle Membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
Tissue Specificity
Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (92%), Bovine (83%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (92%)
Conjugation
Unconjugated
Epitope
Phospho Tyr28
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of MOLT-15 cells treated with phosphatase, using Phospho-LCP1 (Tyr28) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of MOLT-15 cells treated with phosphatase, using Phospho-LCP1 (Tyr28) Antibody.)
IHC (Immunohistochemistry)
(AAA321344 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA321344 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-LCP1 antibody
Function: Actin-binding protein (PubMed:16636079, PubMed:17294403, PubMed:28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed:17294403).
Subunit Structure: Monomer. Interacts with AIF1 (By similarity). Interacts with actin (PubMed:28493397).
Post-translational Modifications: Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
Subunit Structure: Monomer. Interacts with AIF1 (By similarity). Interacts with actin (PubMed:28493397).
Post-translational Modifications: Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 70 kDa
Predicted: 71 kDa
Predicted: 71 kDa
NCBI Official Full Name
plastin-2
NCBI Official Synonym Full Names
lymphocyte cytosolic protein 1
NCBI Official Symbol
LCP1
NCBI Official Synonym Symbols
LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
NCBI Protein Information
plastin-2
UniProt Protein Name
Plastin-2
UniProt Gene Name
LCP1
UniProt Synonym Gene Names
PLS2; LCP-1
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Product Notes
The LCP1 lcp1 (Catalog #AAA321344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-LCP1 (Tyr28) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LCP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LCP1 lcp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LCP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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