HEK293 Whole Cell Lysate
(LDHA was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with AAA200423 with 1:200 dilution.Western blot was performed using AAA200423 at 1/1000 dilution.Lane 1: Control IP in HEK293 Whole Cell Lysate.Lane 2: LDHA IP with AAA200423 in HEK293 Whole Cell Lysate.Lane 3: Input of HEK293 Whole Cell Lysate.)
Rabbit LDHA Polyclonal Antibody | anti-LDHA antibody
LDHA Antibody - middle region
Gene Names
LDHA; LDHM; GSD11; PIG19; HEL-S-133P
Reactivity
Tested Species Reactivity: HumanPredicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Applications
Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Synonyms
LDHA, Antibody; LDHA Antibody - middle region; anti-LDHA antibody
Host
Rabbit
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/mL (varies by lot)
Sequence
Synthetic peptide located within the following region: PLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVH
Sequence Length
332
Applicable Applications for anti-LDHA antibody
IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry)
Homology
Cow: 79%; Dog: 86%; Goat: 79%; Guinea Pig: 79%; Horse: 79%; Human: 100%; Mouse: 86%; Rabbit: 86%; Rat: 79%; Zebrafish: 92%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human LDHA
Protein Size (# AA)
332 amino acids
Protein Interactions
MAPK10; LDHB; FUS; HUWE1; ISG15; UBC; SUMO2; SUMO3; STAU1; RPA3; RPA2; RPA1; ASB9; RNF2; CNDP2; NAGK; C11orf58; NDRG1; BZW1; KYNU; TCEA1; SORD; SMS; RPS6KA1; PGD; PAFAH1B3; GSR; PDIA3; GNAI3; DLD; CSE1L; LDHAL6B; FTO; BCCIP; XRN1; FBXO6; UPF2; YWHAQ; METT
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
HEK293 Whole Cell Lysate
(LDHA was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with AAA200423 with 1:200 dilution.Western blot was performed using AAA200423 at 1/1000 dilution.Lane 1: Control IP in HEK293 Whole Cell Lysate.Lane 2: LDHA IP with AAA200423 in HEK293 Whole Cell Lysate.Lane 3: Input of HEK293 Whole Cell Lysate.)
HEK293 Whole Cell Lysate
(LDHA was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with AAA200423 with 1:200 dilution.Western blot was performed using AAA200423 at 1/1000 dilution.Lane 1: Control IP in HEK293 Whole Cell Lysate.Lane 2: LDHA IP with AAA200423 in HEK293 Whole Cell Lysate.Lane 3: Input of HEK293 Whole Cell Lysate.)
WB (Western Blot)
(Host: RabbitTarget Name: LDHASample Tissue: Human Jurkat, MCF7, 721_BAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: LDHASample Tissue: Human Jurkat, MCF7, 721_BAntibody Dilution: 1.0ug/ml)
IHC (Immunohistochemistry)
(Rabbit Anti-LDHA AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
IHC (Immunohistochemistry)
(Rabbit Anti-LDHA AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-LDHA antibody
This is a rabbit polyclonal antibody against LDHA. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Target Description: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Product Categories/Family for anti-LDHA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37kDa
NCBI Official Full Name
L-lactate dehydrogenase A chain isoform 1
NCBI Official Synonym Full Names
lactate dehydrogenase A
NCBI Official Symbol
LDHA
NCBI Official Synonym Symbols
LDHM; GSD11; PIG19; HEL-S-133P
NCBI Protein Information
L-lactate dehydrogenase A chain
UniProt Protein Name
L-lactate dehydrogenase A chain
UniProt Gene Name
LDHA
UniProt Synonym Gene Names
LDH-A; LDH-M
UniProt Entry Name
LDHA_HUMAN
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Product Notes
The LDHA ldha (Catalog #AAA200423) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LDHA Antibody - middle region reacts with Tested Species Reactivity: Human Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's LDHA can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the LDHA ldha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: PLSCHGWVLG EHGDSSVPVW SGMNVAGVSL KTLHPDLGTD KDKEQWKEVH. It is sometimes possible for the material contained within the vial of "LDHA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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