Rabbit LDHB Polyclonal Antibody | anti-LDHB antibody

Anti-LDHB Antibody

Cat. Num. AAA46838

• Gene Names: LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: LDHB, Antibody; Anti-LDHB Antibody; HEL S 281; LDHB; LDH-B; LDH B; LDHH; LDH-H; LDH H; LDHBD; TRG-5; TRG5; P07195; L-lactate dehydrogenase B chain; lactate dehydrogenase B; anti-LDHB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 334

• Applicable Applications for anti-LDHB antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Notes: Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemistry)

(LDHB was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemisry)

(LDHB was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohiostchemistry)

(LDHB was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot)

(Western blot analysis of LDHB expression in rat brain extract (lane 1), NIH3T3 whole cell lysates (lane 2) and U2OS whole cell lysates (lane 3). LDHB at 37KD was detected using rabbit anti- LDHB Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

Related Product Information for anti-LDHB antibody

Rabbit IgG polyclonal antibody for L-lactate dehydrogenase B chain(LDHB) detection.
Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

References

1. Markert, C. L. Lactate dehydrogenase: biochemistry and function of lactate dehydrogenase. Cell Biochem. Func. 2: 131-134, 1984.
2. Okumura, N., Terasawa, F., Ueno, I., Oki, K., Yamauchi, K., Hidaka, H., Tozuka, M., Okura, M., Katsuyama, T. Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit: LD-B Matsumoto I and II (LD-B W323R). Clin. Chim. Acta 287: 163-171, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 291575128
• NCBI GeneID: 3945
• NCBI Accession #: NP_001167568.1
• NCBI GenBank Nucleotide #: NM_001174097.2
• UniProt Accession #: P07195
• Molecular Weight: 36,638 Da
• NCBI Official Full Name: L-lactate dehydrogenase B chain isoform LDHB
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: LDHB
• NCBI Official Synonym Symbols: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• UniProt Gene Name: LDHB
• UniProt Synonym Gene Names: LDH-B; LDH-H