Rabbit LDLR Polyclonal Antibody | anti-LDLR antibody

LDLR Antibody (Center)

Cat. Num. AAA284656

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Human, Mouse; (Predicted: Xenopus, Bovine, Hamster, Pig, Rabbit, Rat)
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: LDLR, Antibody; LDLR Antibody (Center); Low-density lipoprotein receptor; LDL receptor; LDLR; anti-LDLR antibody

• Host: Rabbit
• Reactivity: Human, Mouse; (Predicted: Xenopus, Bovine, Hamster, Pig, Rabbit, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This LDLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-419 amino acids of human LDLR.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 391-419
• Sequence Length: 860

• Applicable Applications for anti-LDLR antibody: FCM/FACS (Flow Cytometry), WB (Western Blot)
• Function: Binds low density lipoprotein /LDL, the major cholesterol- carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization (PubMed:30443021).
• Cellular Location: Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P01131}. Membrane, clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome Note=Rapidly endocytosed upon ligand binding. Localized at cell membrane, probably in lipid rafts, in serum-starved conditions (PubMed:30443021).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(LDLR Antibody (Center) (AAA284656) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of LDLR Antibody (Center) (AAA284656) in mouse lung tissue lysates (35ug/lane). LDLR (arrow) was detected using the purified Pab.)

Related Product Information for anti-LDLR antibody

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place.

Product Categories/Family for anti-LDLR antibody

Cancer; Cardiovascular; Metabolism; Signal transduction

References

Hobbs,H.H., et.al., Hum. Mutat. 1 (6), 445-466 (1992)
Brown,M.S. et.al., Proc. Natl. Acad. Sci. U.S.A. 76 (7), 3330-3337 (1979)

NCBI and Uniprot Product Information

• NCBI GI #: 4504975
• NCBI GeneID: 3949
• NCBI Accession #: NP_000518.1; NP_001182727.1; NP_001182728.1; NP_001182729.1; NP_001182732.1
• NCBI GenBank Nucleotide #: NM_000527.4
• UniProt Accession #: P01130; Q53ZD9; Q59FQ1; Q9UDH7; B4DII3; B4DJZ8; B4DR00; B4DTQ3; C0JYY8; H0YLU8; H0YNT7
• NCBI Official Full Name: low-density lipoprotein receptor isoform 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor
• UniProt Protein Name: Low-density lipoprotein receptor
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN