WB (Western Blot)
(Anti-LGMN rabbit polyclonal antibody at 1:500 dilutionLane A: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa)
Rabbit anti-Mouse Legumain Polyclonal Antibody | anti-LGMN antibody
Anti-Legumain Antibody, Rabbit Polyclonal
Gene Names
Lgmn; AEP; Prsc1; AI746452; AU022324
Reactivity
Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Legumain, Antibody; Anti-Legumain Antibody, Rabbit Polyclonal; Legumain/LGMN Antibody, Rabbit PAb, Antigen Affinity Purified; legumain; Anti-AEP Antibody; Anti-AI746452 Antibody; Anti-AU022324 Antibody; Anti-Prsc1 Antibody; anti-LGMN antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Legumain/LGMN
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LGMN antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Mouse Legumain/LGMN Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Legumain / LGMN (rM Legumain / LGMN; (Catalog# please inquire); NP_035305.1; Val18-Tyr435). Legumain / LGMN specific IgG was purified by Mouse Legumain / LGMN affinity chromatography
Preparation and Storage
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity.
Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Avoid repeated freeze-thaw cycles
Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Avoid repeated freeze-thaw cycles
WB (Western Blot)
(Anti-LGMN rabbit polyclonal antibody at 1:500 dilutionLane A: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa)
WB (Western Blot)
(Anti-LGMN rabbit polyclonal antibody at 1:500 dilutionLane A: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa)
IHC (Immunohistochemistry)
(Immunochemical staining of mouse LGMN in mouse kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of mouse LGMN in mouse kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
References
Schwarz G, et al. (2002) Characterization of legumain. Biol Chem. 383(11): 1813-6.Liu C, et al. (2003) Overexpression of legumain in tumors is significant for invasion/metastasis and a candidate enzymatic target for prodrug therapy. Cancer Res. 63(11): 2957-64.Murthy RV, et al. (2005) Legumain expression in relation to clinicopathologic and biological variables in colorectal cancer. Clin Cancer Res. 11(6): 2293-9.Gawenda J, et al. (2007) Legumain expression as a prognostic factor in breast cancer patients. Breast Cancer Res Treat. 102(1): 1-6.Clerin V, et al. (2007) Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis. 201(1): 53-66.Lew?"n S, et al. (2008) A Legumain-based minigene vaccine targets the tumor stroma and suppresses breast cancer growth and angiogenesis. Cancer Immunol Immunother. 57(4): 507-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
legumain
NCBI Official Synonym Full Names
legumain
NCBI Official Symbol
Lgmn
NCBI Official Synonym Symbols
AEP; Prsc1; AI746452; AU022324
NCBI Protein Information
legumain
UniProt Protein Name
Legumain
UniProt Gene Name
Lgmn
UniProt Synonym Gene Names
Prsc1
UniProt Entry Name
LGMN_MOUSE
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Product Notes
The LGMN lgmn (Catalog #AAA257070) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Legumain Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Legumain can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LGMN lgmn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Legumain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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