WB (Western Blot)
(Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human LEO1 Polyclonal Antibody | anti-LEO1 antibody
LEO1 Antibody (N-term)
Gene Names
LEO1; RDL
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LEO1, Antibody; LEO1 Antibody (N-term); RNA polymerase-associated protein LEO1; Replicative senescence down-regulated leo1-like protein; LEO1; RDL; anti-LEO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LEO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the N-terminal region of human LEO1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
130-159
Sequence Length
606
Applicable Applications for anti-LEO1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of anti-Leo1 Antibody (N-term) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-Leo1 Antibody (N-term) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.)
Related Product Information for anti-LEO1 antibody
The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.
References
Zhao,L., FASEB J. 19 (6), 521-532 (2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75404
NCBI Official Full Name
RNA polymerase-associated protein LEO1 isoform 1
NCBI Official Synonym Full Names
Leo1 Paf1/RNA polymerase II complex component
NCBI Official Symbol
LEO1
NCBI Official Synonym Symbols
RDL
NCBI Protein Information
RNA polymerase-associated protein LEO1
UniProt Protein Name
RNA polymerase-associated protein LEO1
UniProt Gene Name
LEO1
UniProt Synonym Gene Names
RDL
UniProt Entry Name
LEO1_HUMAN
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Product Notes
The LEO1 leo1 (Catalog #AAA290346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 130-159. The LEO1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LEO1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LEO1 leo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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