Rabbit anti-Human Leptin Receptor Polyclonal Antibody | anti-LEPR antibody

Anti-Leptin Receptor Antibody, Rabbit Polyclonal

Cat. Num. AAA259487

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Leptin Receptor, Antibody; Anti-Leptin Receptor Antibody, Rabbit Polyclonal; Leptin Receptor Antibody, Rabbit PAb, Antigen Affinity Purified; leptin receptor; Anti-CD295 Antibody; Anti-DB Antibody; Anti-LEP-R Antibody; Anti-LEPRD Antibody; Anti-OB-R Antibody; Anti-OBR Antibody; anti-LEPR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Leptin Receptor
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LEPR antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant Human Leptin Receptor Protein ( NP_002294.2; Met1-Asp839). Leptin Receptor specific IgG was purified by Human Leptin Receptor affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IHC (Immunohistochemisry)

(Immunochemical staining of human Leptin Receptor in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human Leptin Receptor in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-Leptin Receptor rabbit polyclonal antibody at 1:500 dilutionLane A: U87MG Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:127 kDaObserved band size:127 kDa)

Related Product Information for anti-LEPR antibody

Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight) and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra-Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K, and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in the active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in the Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.

References

Heshk. JT. e. al. (2001. . rol. fo. dietar. fa. i. lepti. receptor. OB-Rb. function. Lif. Sci. 6. (9). 987-1003.Che. H. e. a. (1996. Evidenc. tha. th. diabete. gen. encode. th. lepti. receptor. identificatio. o. . mutatio. i. th. lepti. recepto. gen. i. db/d. mice. Cell. 8. (3). 491-5.Bjrbae. C. e. al. (1998. Divergen. signalin. capacitie. o. th. lon. an. shor. isoform. o. th. lepti. receptor. . Bio. Chem. 27. (51). 32686-95.

NCBI and Uniprot Product Information

• NCBI GI #: 40254464
• NCBI GeneID: 3953
• NCBI Accession #: NP_002294.2
• NCBI GenBank Nucleotide #: NM_002303.5
• UniProt Accession #: P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921
• Molecular Weight: 96,042 Da
• NCBI Official Full Name: leptin receptor isoform 1
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor; huB219; OB receptor
• UniProt Protein Name: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN