WB (Western Blot)
(Anti-LTA4H rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:63 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Leukotriene A4 Hydrolase Polyclonal Antibody | anti-LTA4H antibody
Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Leukotriene A4 Hydrolase, Antibody; Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Polyclonal; Leukotriene A4 Hydrolase/LTA4H Antibody, Rabbit PAb, Antigen Affinity Purified; leukotriene A4 hydrolase; Anti-LAT4 Antibody; anti-LTA4H antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Leukotriene A4 Hydrolase/LTA4H
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LTA4H antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Leukotriene A4 Hydrolase/LTA4H protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Leukotriene A4 Hydrolase/LTA4H (rh Leukotriene A4 Hydrolase/LTA4H). Leukotriene A4 Hydrolase/LTA4H specific IgG was purified by Human Leukotriene A4 Hydrolase/LTA4H affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-LTA4H rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:63 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-LTA4H rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 60 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:70 kDaObserved band size:63 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(LTA4H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LTA4H rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size: 69 kDa)
IP (Immunoprecipitation)
(LTA4H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate2 uL anti-LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LTA4H rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size: 69 kDa)
Related Product Information for anti-LTA4H antibody
Leukotriene A-4 hydrolase, also known as LTA-4 hydrolase, Leukotriene A (4) hydrolase, LTA4H, and LTA4, is a cytoplasm protein that belongs to the peptidase M1 family. LTA4H hydrolyzes an epoxide moiety of leukotriene A4 (LTA-4) to form leukotriene B4 (LTB-4). This enzyme also has some peptidase activity. The leukotrienes (LTs) are a class of structurally related lipid mediators involved in the development and maintenance of inflammatory and allergic reactions. In the biosynthesis of LTs, arachidonic acid was converted into the unstable intermediate epoxide LTA4, which may, in turn, be conjugated with glutathione to form the spasmogenic LTC4, or hydrolyzed into the pro-inflammatory lipid mediator LTB4 in a reaction catalyzed by Leukotriene A4 hydrolase (LTA4H). LTB4 is a classical chemoattractant of human neutrophils and triggers adherence and aggregation of leukocytes to vascular endothelium, and also modulates immune responses. As a bifunctional zinc metalloenzyme, LTA4H also exhibits an anion-dependant arginyl aminopeptidase activity of high efficiency and specificity in addition to its epoxide hydrolase activity. LTA4H is regarded as a therapeutic target for inflammation.
References
Mancini, JA. et al., 1995, Eur. J. Biochem. 231: 65-71. Orning, L. et al., 1994, J. Biol. Chem. 269: 11269-73. Rudberg, PC. et al., 2004, J. Biol. Chem. 279: 27376-82. Qiu, H. et al., 2006, Proc. Natl. Acad. Sci. USA. 103: 8161-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,852 Da
NCBI Official Full Name
leukotriene A-4 hydrolase isoform 1
NCBI Official Synonym Full Names
leukotriene A4 hydrolase
NCBI Official Symbol
LTA4H
NCBI Protein Information
leukotriene A-4 hydrolase
UniProt Protein Name
Leukotriene A-4 hydrolase
UniProt Gene Name
LTA4H
UniProt Synonym Gene Names
LTA4; LTA-4 hydrolase
UniProt Entry Name
LKHA4_HUMAN
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Product Notes
The LTA4H lta4h (Catalog #AAA254783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Leukotriene A4 Hydrolase can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LTA4H lta4h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leukotriene A4 Hydrolase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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