Rabbit Leukotriene B4 Receptor / BLT1 Polyclonal Antibody | anti-LTB4R antibody

Rabbit Polyclonal to Human Leukotriene B4 Receptor / BLT1

Cat. Num. AAA51624

• Gene Names: LTB4R; BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
• Reactivity: Human; Predicted Reactivity: Monkey, Mouse, Rat, Hamster, Rabbit
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: Leukotriene B4 Receptor / BLT1, Antibody; Rabbit Polyclonal to Human Leukotriene B4 Receptor / BLT1; Anti-Leukotriene B4 Receptor / BLT1 Antibody (Cytoplasmic Domain) IHC-plus; BLT; BLTR; Blt-1; Chemokine receptor-like 1; G protein-coupled receptor 16; GPR16; Leukotriene B4 receptor; Leukotriene B4 receptor 1; G-protein coupled receptor 16; LTBR1; p2Y purinoceptor 7; p2Y7; LTB4-R 1; p2RY7; CMKRL1; LTB4-R1; LTB4R1; Human Leukotriene B4 Receptor; BLT1; anti-LTB4R antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Monkey, Mouse, Rat, Hamster, Rabbit
• Clonality: Polyclonal
• Specificity: Human Leukotriene B4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 352

• Applicable Applications for anti-LTB4R antibody: IHC (Immunohistochemistry)
• Immunogen Species: Raised against human
• Antigen Type: Synthetic peptide
• Immunogen: Leukotriene B4 Receptor / BLT1 antibody was raised against synthetic 20 amino acid peptide from 1st cytoplasmic domain of human Leukotriene B4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Rat, Rabbit (95%); Monkey, Mouse, Hamster (90%).
• Epitope: Cytoplasmic Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year.; Can be stored undiluted at 4°C for up one month.; Avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(Anti-Leukotriene B4 Receptor antibody IHC of human spleen, red pulp. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-LTB4R antibody

BLT1 is a Chemoattractant Receptor that binds the potent chemoattractant leukotriene B4 (LTB4) which is involved in defense against infection. Leukotriene B4 also activates the transcription factor PPAR alpha, resulting in the activation of genes that terminate inflammatory processes (Yokomizo et al., 1997). Although BLT1 shows homology to other P2Y receptors, it does not function as a purinoreceptor (Yokomizo et al., 1997).

Product Categories/Family for anti-LTB4R antibody

Family: GPCR; Subfamily: Chemoattractant

NCBI and Uniprot Product Information

• NCBI GI #: 221218998
• NCBI GeneID: 1241
• NCBI Accession #: NP_001137391.1
• NCBI GenBank Nucleotide #: NM_001143919.2
• UniProt Accession #: Q15722; Q13305; Q53XV5; Q92641; Q9BSU5
• NCBI Official Full Name: leukotriene B4 receptor 1
• NCBI Official Synonym Full Names: leukotriene B4 receptor
• NCBI Official Symbol: LTB4R
• NCBI Official Synonym Symbols: BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
• NCBI Protein Information: leukotriene B4 receptor 1; LTB4-R1; LTB4-R 1; P2Y purinoceptor 7; chemokine receptor-like 1; G protein-coupled receptor 16; G-protein coupled receptor 16; chemoattractant receptor-like 1; purinergic receptor P2Y, G-protein coupled, 7
• UniProt Protein Name: Leukotriene B4 receptor 1
• UniProt Gene Name: LTB4R
• UniProt Synonym Gene Names: BLT; BLT1; BLTR; CMKRL1; GPR16; P2RY7; LTB4-R 1; LTB4-R1; P2Y7
• UniProt Entry Name: LT4R1_HUMAN