WB (Western Blot)
(LIMK1 Antibody (T9) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).)
Rabbit LIM Kinase 1 (LIMK1) Polyclonal Antibody | anti-LIMK1 antibody
LIM Kinase 1 (LIMK1) Antibody (N-term)
Gene Names
LIMK1; LIMK; LIMK-1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LIM Kinase 1 (LIMK1), Antibody; LIM Kinase 1 (LIMK1) Antibody (N-term); LIM domain kinase 1; LIMK-1; LIMK1; LIMK; anti-LIMK1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LIM Kinase 1 (LIMK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIM Kinase 1 (LIMK1).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
1-30
Sequence Length
613
Applicable Applications for anti-LIMK1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(LIMK1 Antibody (T9) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).)
WB (Western Blot)
(LIMK1 Antibody (T9) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-LIMK1 antibody
LIMK1, a member of the Ser/Thr protein kinase family, may be a component of an intracellular signaling pathway and may be involved in brain development. It phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1, and this cytoplasmic protein also binds ROCK1, whic phosphorylates LIMK1 on serine and/or threonine residues. Highest expression occurs in both adult and fetal nervous systems. It is detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex, and is expressed to a lesser extent in heart and skeletal muscle. Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. This protein contains 2 LIM zinc-binding domains and 1 PDZ/DHR domain.
Product Categories/Family for anti-LIMK1 antibody
References
Ohashi, K., et al., J. Biol. Chem. 275(5):3577-3582 (2000).
Maekawa, M., et al., Science 285(5429):895-898 (1999).
Edwards, D.C., et al., J. Biol. Chem. 274(16):11352-11361 (1999).
Osborne, L.R., et al., Genomics 36(2):328-336 (1996).
Frangiskakis, J.M., et al., Cell 86(1):59-69 (1996).
Maekawa, M., et al., Science 285(5429):895-898 (1999).
Edwards, D.C., et al., J. Biol. Chem. 274(16):11352-11361 (1999).
Osborne, L.R., et al., Genomics 36(2):328-336 (1996).
Frangiskakis, J.M., et al., Cell 86(1):59-69 (1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72585
NCBI Official Full Name
LIM domain kinase 1 isoform 2
NCBI Official Synonym Full Names
LIM domain kinase 1
NCBI Official Symbol
LIMK1
NCBI Official Synonym Symbols
LIMK; LIMK-1
NCBI Protein Information
LIM domain kinase 1
UniProt Protein Name
LIM domain kinase 1
UniProt Gene Name
LIMK1
UniProt Synonym Gene Names
LIMK; LIMK-1
UniProt Entry Name
LIMK1_HUMAN
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Product Notes
The LIMK1 limk1 (Catalog #AAA286553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The LIM Kinase 1 (LIMK1) Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's LIM Kinase 1 (LIMK1) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the LIMK1 limk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIM Kinase 1 (LIMK1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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