IF (Immunofluorescence)
(Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.)
Rabbit anti-Human LIMA1 Polyclonal Antibody | anti-LIMA1 antibody
LIMA1 Antibody
Gene Names
LIMA1; EPLIN; SREBP3
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
LIMA1 antibody is affinity chromatography purified via peptide column.
Synonyms
LIMA1, Antibody; LIMA1 Antibody; LIM domain and actin-binding protein 1; PP624; EPLIN; SREBP3 ; LIM domain and actin binding 1; anti-LIMA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Purity/Purification
LIMA1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
760
Applicable Applications for anti-LIMA1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1
Buffer
LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
LIMA1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 μg/ml.)
Related Product Information for anti-LIMA1 antibody
The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 50; 66; 84 kDa
Observed: 65 kDa
Observed: 65 kDa
NCBI Official Full Name
LIM domain and actin-binding protein 1 isoform 1
NCBI Official Synonym Full Names
LIM domain and actin binding 1
NCBI Official Symbol
LIMA1
NCBI Official Synonym Symbols
EPLIN; SREBP3
NCBI Protein Information
LIM domain and actin-binding protein 1; 1110021C24Rik; epithelial protein lost in neoplasm beta; sterol regulatory element binding protein 3
UniProt Protein Name
LIM domain and actin-binding protein 1
UniProt Gene Name
LIMA1
UniProt Synonym Gene Names
EPLIN; SREBP3
UniProt Entry Name
LIMA1_HUMAN
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Product Notes
The LIMA1 lima1 (Catalog #AAA40563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIMA1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIMA1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the LIMA1 lima1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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