WB (Western Blot)
(Detection of human LIN28B by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-LIN28B antibody AAA212232 (lot AAA212232-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human LIN28B Polyclonal Antibody | anti-LIN28B antibody
Rabbit anti-LIN28B Antibody, Affinity Purified
Gene Names
LIN28B; CSDD2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
LIN28B, Antibody; Rabbit anti-LIN28B Antibody, Affinity Purified; CSDD2; Lin-28.2; lin-28B; protein lin-28 homolog B; Protein lin-28 homolog B; lin-28 homolog B; anti-LIN28B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-LIN28B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human LIN28B by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-LIN28B antibody AAA212232 (lot AAA212232-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human LIN28B by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (H; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-LIN28B antibody AAA212232 (lot AAA212232-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human LIN28B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-LIN28B antibody AAA212232 (lot AAA212232-1) used for IP at 6 ug/mg lysate. LIN28B was also immunoprecipitated by rabbit anti-LIN28B antibody which recognizes a downstream epitope. For blotting immunoprecipitated LIN28B, AAA212232 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human LIN28B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-LIN28B antibody AAA212232 (lot AAA212232-1) used for IP at 6 ug/mg lysate. LIN28B was also immunoprecipitated by rabbit anti-LIN28B antibody which recognizes a downstream epitope. For blotting immunoprecipitated LIN28B, AAA212232 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
Related Product Information for anti-LIN28B antibody
Lin-28 homolog B (LIN28B) acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28B acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. LIN28B specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. It also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6ZN17].
Product Categories/Family for anti-LIN28B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,511 Da
NCBI Official Full Name
protein lin-28 homolog B
NCBI Official Synonym Full Names
lin-28 homolog B (C. elegans)
NCBI Official Symbol
LIN28B
NCBI Official Synonym Symbols
CSDD2
NCBI Protein Information
protein lin-28 homolog B; Lin-28.2; lin-28B
UniProt Protein Name
Protein lin-28 homolog B
UniProt Gene Name
LIN28B
UniProt Synonym Gene Names
CSDD2; Lin-28B
UniProt Entry Name
LN28B_HUMAN
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Product Notes
The LIN28B lin28b (Catalog #AAA212232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LIN28B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIN28B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LIN28B lin28b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIN28B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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