IHC (Immunohistochemisry)
(AAA324806 at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)
Rabbit anti-Human LIPI Polyclonal Antibody | anti-LIPI antibody
LIPI Antibody
Gene Names
LIPI; CT17; LPDL; PLA1C; PRED5; mPA-PLA1 beta
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
LIPI, Antibody; LIPI Antibody; Cancer/testis antigen 17; CT17; Lipase member I; LIPI; LIPI_HUMAN; LPD lipase; LPDL; Membrane-associated phosphatidic acid-selective phospholipase A1-beta; membrane-associated phospholipase A1 beta 7B+; membrane-associated phospholipase A1 beta deltaE2-3; membrane-associated phospholipase A1 beta deltaE5; membrane-associated phospholipase A1 beta deltaE6.1; membrane-associated phospholipase A1 beta deltaE7.2; membrane-associated phospholipase A1 beta deltaE8-9; membrane-associated phospholipase A1 beta fl; mPA-PLA1 beta; PLA1C; PRED5; anti-LIPI antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
460
Applicable Applications for anti-LIPI antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
A synthesized peptide derived from human LIPI, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IHC (Immunohistochemisry)
(AAA324806 at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)
IHC (Immunohistochemisry)
(AAA324806 at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.)
IF (Immunofluorescence)
(AAA324806 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab AAA324806 and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA324806 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab AAA324806 and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from COLO cells, using LIPI antibody., The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from COLO cells, using LIPI antibody., The lane on the left is treated with the antigen-specific peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53 KD
NCBI Official Full Name
lipase member I isoform 1
NCBI Official Synonym Full Names
lipase I
NCBI Official Symbol
LIPI
NCBI Official Synonym Symbols
CT17; LPDL; PLA1C; PRED5; mPA-PLA1 beta
NCBI Protein Information
lipase member I
UniProt Protein Name
Lipase member I
UniProt Gene Name
LIPI
UniProt Synonym Gene Names
LPDL; LIPI; CT17; mPA-PLA1 beta
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Product Notes
The LIPI lipi (Catalog #AAA324806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIPI Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIPI can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the LIPI lipi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIPI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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