Goat LMNB1 / Lamin B1 Polyclonal Antibody | anti-LMNB1 antibody

Goat Polyclonal to Human LMNB1 / Lamin B1

Cat. Num. AAA52470

• Gene Names: LMNB1; LMN; ADLD; LMN2; LMNB
• Reactivity: Human , Mouse , Bovine, Dog , Pig
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium precipitation followed by antigen affinity chromatograpy using the immunizing peptide.
• Synonyms: LMNB1 / Lamin B1, Antibody; Goat Polyclonal to Human LMNB1 / Lamin B1; Anti-LMNB1 / Lamin B1 Antibody (aa526-537) IHC-plus; LMNB1; ADLD; Lamin B1; Lamin-B1; LMN; LMNB; LMN2; Human LMNB1; anti-LMNB1 antibody

• Host: Goat
• Reactivity: Human , Mouse , Bovine, Dog , Pig
• Clonality: Polyclonal
• Specificity: Human LMNB1 / Lamin B1. This antibody is expected to recognize both reported isoforms (NP_005564.1; NP_001185486.1).
• Purity/Purification: Purified from goat serum by ammonium precipitation followed by antigen affinity chromatograpy using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 586

• Applicable Applications for anti-LMNB1 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Immunogen Species: LMNB1 / Lamin B1 antibody was raised against Human
• Immunogen: LMNB1 / Lamin B1 antibody was raised against synthetic peptide with sequence C-DVKVILKNSQGE from an internal region (near C-terminus) of the protein sequence according to (NP_005564.1; NP_001185486.1).
• Epitope: aa526-537
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20 degree C. Avoid freeze-thaw cycles.

WB (Western Blot)

(LMNB1 antibody (1 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry)

(Anti-LMNB1 / Lamin B1 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 5031877
• NCBI GeneID: 4001
• NCBI Accession #: NP_005564.1
• NCBI GenBank Nucleotide #: NM_005573.3
• UniProt Accession #: P20700; Q3SYN7; Q96EI6; B2R6J6
• NCBI Official Full Name: lamin-B1 isoform 1
• NCBI Official Synonym Full Names: lamin B1
• NCBI Official Symbol: LMNB1
• NCBI Official Synonym Symbols: LMN; ADLD; LMN2; LMNB
• NCBI Protein Information: lamin-B1
• UniProt Protein Name: Lamin-B1
• UniProt Gene Name: LMNB1
• UniProt Synonym Gene Names: LMN2; LMNB
• UniProt Entry Name: LMNB1_HUMAN