WB (Western Blot)
(LOXL2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LOXL2 antibody detected the LOXL2 protein (arrow).)
Rabbit LOXL2 Polyclonal Antibody | anti-LOXL2 antibody
LOXL2 Antibody (C-term)
Gene Names
LOXL2; LOR2; WS9-14
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Applications
ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
LOXL2, Antibody; LOXL2 Antibody (C-term); Lysyl oxidase homolog 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; LOXL2; anti-LOXL2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This LOXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 589-617 amino acids from the C-terminal region of human LOXL2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
589-617
Sequence Length
774
Applicable Applications for anti-LOXL2 antibody
ELISA, WB (Western Blot)
Function
Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) (PubMed:27735137). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation (PubMed:27735137). Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2) (PubMed:27735137). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription (PubMed:25959397). LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2 (By similarity). Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin CDH1, probably by mediating deamination of histone H3 (PubMed:16096638, PubMed:24414204, PubMed:27735137). During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription (PubMed:24239292). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (PubMed:24239292). Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction (PubMed:28332555). Involved in E-cadherin repression following hypoxia, a hallmark of EMT believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression (PubMed:20026874). When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed:20306300). Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding (PubMed:21835952). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation (By similarity).
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus. Chromosome. Endoplasmic reticulum. Note=Associated with chromatin (PubMed:27735137). It is unclear how LOXL2 is nuclear as it contains a signal sequence and has been shown to be secreted (PubMed:23319596) However, a number of reports confirm its intracellular location and its key role in transcription regulation (PubMed:22204712, PubMed:22483618).
Tissue Location
Expressed in many tissues (PubMed:10212285). Highest expression in reproductive tissues, placenta, uterus and prostate (PubMed:10212285). In esophageal epithelium, expressed in the basal, prickle and granular cell layers (PubMed:22204712). Up-regulated in a number of cancers cells and tissues.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(LOXL2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LOXL2 antibody detected the LOXL2 protein (arrow).)
WB (Western Blot)
(LOXL2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the LOXL2 antibody detected the LOXL2 protein (arrow).)
Related Product Information for anti-LOXL2 antibody
This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.
Product Categories/Family for anti-LOXL2 antibody
References
Rodriguez, H.M., et al. J. Biol. Chem. 285(27):20964-20974(2010)
Ruckert, F., et al. Int J Colorectal Dis 25(3):303-311(2010)
Schietke, R., et al. J. Biol. Chem. 285(9):6658-6669(2010)
Sano, M., et al. Int. J. Oncol. 36(2):321-330(2010)
Kim, Y., et al. Oncol. Rep. 22(4):799-804(2009)
Ruckert, F., et al. Int J Colorectal Dis 25(3):303-311(2010)
Schietke, R., et al. J. Biol. Chem. 285(9):6658-6669(2010)
Sano, M., et al. Int. J. Oncol. 36(2):321-330(2010)
Kim, Y., et al. Oncol. Rep. 22(4):799-804(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 86725
NCBI Official Full Name
lysyl oxidase homolog 2
NCBI Official Synonym Full Names
lysyl oxidase-like 2
NCBI Official Symbol
LOXL2
NCBI Official Synonym Symbols
LOR2; WS9-14
NCBI Protein Information
lysyl oxidase homolog 2
UniProt Protein Name
Lysyl oxidase homolog 2
UniProt Gene Name
LOXL2
UniProt Entry Name
LOXL2_HUMAN
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Product Notes
The LOXL2 loxl2 (Catalog #AAA289086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 589-617. The LOXL2 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's LOXL2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LOXL2 loxl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOXL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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