IHC (Immunohiostchemistry)
(AAA328945 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Lubricin Polyclonal Antibody | anti-PRG4 antibody
Lubricin Antibody
Gene Names
PRG4; MSF; SZP; CACP; HAPO; JCAP
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
Peptide affinity purification.
Synonyms
Lubricin, Antibody; Lubricin Antibody; Articular superficial zone protein; bG174L6.2; CACP; Camptodactyly arthropathy coxa vara pericarditis syndrome gene; FLJ32635; HAPO; Jacobs camptodactyly arthropathy pericarditis syndrome gene; JCAP; Megakaryocyte stimulating factor; MSF; PRG 4; PRG4; Proteoglycan 4; Proteoglycan4; Superficial zone proteoglycan; SZP; anti-PRG4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Lubricin Antibody detects endogenous levels of total Lubricin.
Purity/Purification
Peptide affinity purification.
Form/Format
Phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
1363
Applicable Applications for anti-PRG4 antibody
ELISA
Immunogen
A synthesized peptide derived from human Lubricin, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
IMPORTANT
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA328945 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328945 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Human liver, using Lubricin Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Human liver, using Lubricin Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PRG4 antibody
Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
151 kDa
NCBI Official Full Name
proteoglycan 4 isoform B preproprotein
NCBI Official Synonym Full Names
proteoglycan 4
NCBI Official Symbol
PRG4
NCBI Official Synonym Symbols
MSF; SZP; CACP; HAPO; JCAP
NCBI Protein Information
proteoglycan 4
UniProt Protein Name
Proteoglycan 4
UniProt Gene Name
PRG4
UniProt Synonym Gene Names
MSF; SZP
Similar Products
Product Notes
The PRG4 prg4 (Catalog #AAA328945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lubricin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lubricin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the PRG4 prg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lubricin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.