WB (Western Blot)
(LXR-a antibody (pAb) tested by Western blot. Whole cell extract of Hep G2 cells (30 ug) probed with LXR-a antibody (pAb) at a dilution of 1:500.)
Rabbit anti-Human, Mouse LXR-alpha Polyclonal Antibody | anti-LXR-alpha antibody
LXR-alpha antibody (pAb)
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human, Mouse
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
LXR-alpha, Antibody; LXR-alpha antibody (pAb); NR1H3; LXRA; RLD-1; anti-LXR-alpha antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified rabbit IgG in 70mM Tris, 105mM EDTA, 31mM glycine, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-LXR-alpha antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This LXR-a antibody was raised against a peptide in the N-terminal region of human LXR-a.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(LXR-a antibody (pAb) tested by Western blot. Whole cell extract of Hep G2 cells (30 ug) probed with LXR-a antibody (pAb) at a dilution of 1:500.)
WB (Western Blot)
(LXR-a antibody (pAb) tested by Western blot. Whole cell extract of Hep G2 cells (30 ug) probed with LXR-a antibody (pAb) at a dilution of 1:500.)
ChIP (Chromatin Immunoprecipitation)
(LXR-a antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 20 ug of mouse embryonic fibroblast chromatin and 10 ul of LXR-a antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(LXR-a antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 20 ug of mouse embryonic fibroblast chromatin and 10 ul of LXR-a antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(LXR-a antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2 million mouse embryonic fibroblasts. ChIP DNA was amplified by WGA, labeled and hybridized to a mouse tiling array. The image is zoomed in to show LXR-a binding at the promoter of the retinoic acid inducible gene Zmiz1. LXR binding at this gene is expected since LXR is known to form heterodimers with RXR (retinoic acid receptor).)
ChIP (Chromatin Immunoprecipitation)
(LXR-a antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 2 million mouse embryonic fibroblasts. ChIP DNA was amplified by WGA, labeled and hybridized to a mouse tiling array. The image is zoomed in to show LXR-a binding at the promoter of the retinoic acid inducible gene Zmiz1. LXR binding at this gene is expected since LXR is known to form heterodimers with RXR (retinoic acid receptor).)
Related Product Information for anti-LXR-alpha antibody
Short Description: Our LXR-alpha antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human and Mouse samples.
Background: LXR-a (Liver X receptor alpha, NR1H3) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-a and LXR-b) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-a is expressed mainly in liver and adipose tissue and interacts with RXR, shifting RXR to an active ligand-binding subunit mediating responses to retinoids.
Background: LXR-a (Liver X receptor alpha, NR1H3) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-a and LXR-b) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-a is expressed mainly in liver and adipose tissue and interacts with RXR, shifting RXR to an active ligand-binding subunit mediating responses to retinoids.
Product Categories/Family for anti-LXR-alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 44; 49 kDa
Observed: 46 kDa
Observed: 46 kDa
NCBI Official Full Name
oxysterols receptor LXR-alpha isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 1, group H, member 3
NCBI Official Symbol
NR1H3
NCBI Official Synonym Symbols
LXRA; LXR-a; RLD-1
NCBI Protein Information
oxysterols receptor LXR-alpha; liver X nuclear receptor alpha variant 1
UniProt Protein Name
Oxysterols receptor LXR-alpha
UniProt Gene Name
NR1H3
UniProt Synonym Gene Names
LXRA
UniProt Entry Name
NR1H3_HUMAN
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Product Notes
The LXR-alpha nr1h3 (Catalog #AAA59987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LXR-alpha antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LXR-alpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the LXR-alpha nr1h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LXR-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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