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Application Data
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: KDM4A knockout MCF7 cell lysate; Predicted MW: 120,54kd Observed MW: 50kd Primary Ab: 2ug/ml Rabbit Anti-Human KDM4A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human, Mouse Lysine Specific Demethylase 4A Polyclonal Antibody | anti-KDM4A antibody
Polyclonal Antibody to Lysine Specific Demethylase 4A (KDM4A)
Gene Names
KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Lysine Specific Demethylase 4A, Antibody; Polyclonal Antibody to Lysine Specific Demethylase 4A (KDM4A); anti-KDM4A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KDM4A. It has beenselected for its ability to recognize KDM4A in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- YEKHVDEWNI GRLRTILDLV EKESGITIEG VNTPYLYFGM WKTSFAWHTE DMDLYSINYL HFGEPKSWYS VPPEHGKRLE RLAKGFFPGS AQSCEAFLRH KMTLISPLML KKYGIPFDKV TQEAGEFMIT FPYGYHAGFN
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- YEKHVDEWNI GRLRTILDLV EKESGITIEG VNTPYLYFGM WKTSFAWHTE DMDLYSINYL HFGEPKSWYS VPPEHGKRLE RLAKGFFPGS AQSCEAFLRH KMTLISPLML KKYGIPFDKV TQEAGEFMIT FPYGYHAGFN
Sequence Length
1064
Applicable Applications for anti-KDM4A antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
KDM4A (Tyr141~Lys310)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse
Conjugate
No Conjugate
Immunogen
Recombinant KDM4A (Tyr141~Lys310) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Application Data
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: KDM4A knockout MCF7 cell lysate; Predicted MW: 120,54kd Observed MW: 50kd Primary Ab: 2ug/ml Rabbit Anti-Human KDM4A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Application Data
(Knockout Validation: Lane 1: Wild-type MCF7 cell lysate; Lane 2: KDM4A knockout MCF7 cell lysate; Predicted MW: 120,54kd Observed MW: 50kd Primary Ab: 2ug/ml Rabbit Anti-Human KDM4A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue))
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Stomach Tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded lung tissue))
IHC (Immunohistochemisry)
(DAB staining on fromalin fixed paraffin-embedded lung tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Human KDM4A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 2ug/ml Rabbit Anti-Human KDM4A Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-KDM4A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,697 Da
NCBI Official Full Name
lysine-specific demethylase 4A
NCBI Official Synonym Full Names
lysine demethylase 4A
NCBI Official Symbol
KDM4A
NCBI Official Synonym Symbols
JMJD2; JHDM3A; JMJD2A; TDRD14A
NCBI Protein Information
lysine-specific demethylase 4A
UniProt Protein Name
Lysine-specific demethylase 4A
UniProt Gene Name
KDM4A
UniProt Synonym Gene Names
JHDM3A; JMJD2; JMJD2A; KIAA0677
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Product Notes
The KDM4A kdm4a (Catalog #AAA141553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lysine Specific Demethylase 4A (KDM4A) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lysine Specific Demethylase 4A can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KDM4A kdm4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- YEKHVDEWNI GRLRTILDLV EKESGITIEG VNTPYLYFGM WKTSFAWHTE DMDLYSINYL HFGEPKSWYS VPPEHGKRLE RLAKGFFPGS AQSCEAFLRH KMTLISPLML KKYGIPFDKV TQEAGEFMIT FPYGYHAGFN. It is sometimes possible for the material contained within the vial of "Lysine Specific Demethylase 4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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