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product-image-AAA143886_AD8.jpg Application Data (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: KARS knockout Jurkat cell lysate; Predicted MW: 68,71kd Observed MW: 71kd Primary Ab: 3ug/ml Rabbit Anti-Human KARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

Rabbit anti-Human Lysyl tRNA Synthetase Polyclonal Antibody | anti-KARS antibody

Polyclonal Antibody to Lysyl tRNA Synthetase (KARS)

Gene Names
KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Lysyl tRNA Synthetase, Antibody; Polyclonal Antibody to Lysyl tRNA Synthetase (KARS); anti-KARS antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KARS. It has beenselected for its ability to recognize KARS in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
503
Applicable Applications for anti-KARS antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
KARS (Arg323~Arg553)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant KARS (Arg323~Arg553) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Application Data

(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: KARS knockout Jurkat cell lysate; Predicted MW: 68,71kd Observed MW: 71kd Primary Ab: 3ug/ml Rabbit Anti-Human KARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

product-image-AAA143886_AD8.jpg Application Data (Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: KARS knockout Jurkat cell lysate; Predicted MW: 68,71kd Observed MW: 71kd Primary Ab: 3ug/ml Rabbit Anti-Human KARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

product-image-AAA143886_IHC10.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

IHC (Immunohistochemisry)

(DAB staining on IHC-P; Samples: Human Stomach Tissue)

product-image-AAA143886_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Stomach Tissue)

WB (Western Blot)

(Western Blot: Sample: Recombinant KARS, Human.)

product-image-AAA143886_WB13.jpg WB (Western Blot) (Western Blot: Sample: Recombinant KARS, Human.)

WB (Western Blot)

(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human Hela Cells; Lane3: Human A549 Cells.)

product-image-AAA143886_WB15.jpg WB (Western Blot) (Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human Hela Cells; Lane3: Human A549 Cells.)
Product Categories/Family for anti-KARS antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
71,497 Da
NCBI Official Full Name
lysine--tRNA ligase isoform 1
NCBI Official Synonym Full Names
lysyl-tRNA synthetase
NCBI Official Symbol
KARS
NCBI Official Synonym Symbols
KRS; KARS1; KARS2; CMTRIB; DFNB89
NCBI Protein Information
lysine--tRNA ligase
UniProt Protein Name
Lysine--tRNA ligase
UniProt Gene Name
KARS
UniProt Synonym Gene Names
KIAA0070; LysRS

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Product Notes

The KARS kars (Catalog #AAA143886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lysyl tRNA Synthetase (KARS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lysyl tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KARS kars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysyl tRNA Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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