IP (Immunoprecipitation)
(Detection of human MAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MAD2 antibody AAA210872 (lot AAA210872-4) used for IP at 6 ug per reaction. MAD2 was also immunoprecipitated by a previous lot of this antibody (lot AAA210872-3) and rabbit anti-MAD2 antibody For blotting immunoprecipitated MAD2, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time time of 10 seconds.)
Rabbit anti-Human MAD2 Polyclonal Antibody | anti-MAD2 antibody
Rabbit anti-MAD2 Antibody, Affinity Purified
Gene Names
MAD2L1; MAD2; HSMAD2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
MAD2, Antibody; Rabbit anti-MAD2 Antibody, Affinity Purified; MAD2; mitotic arrest deficient 2-like protein 1; mitotic spindle assembly checkpoint protein MAD2A; mitotic arrest deficient, yeast, homolog-like 1; HSMAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2 mitotic arrest deficient-like 1; MAD2-like protein 1; Mitotic spindle assembly checkpoint protein MAD2A; mitotic arrest deficient 2 like 1; anti-MAD2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MAD2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 100 and 150
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human MAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MAD2 antibody AAA210872 (lot AAA210872-4) used for IP at 6 ug per reaction. MAD2 was also immunoprecipitated by a previous lot of this antibody (lot AAA210872-3) and rabbit anti-MAD2 antibody For blotting immunoprecipitated MAD2, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MAD2 antibody AAA210872 (lot AAA210872-4) used for IP at 6 ug per reaction. MAD2 was also immunoprecipitated by a previous lot of this antibody (lot AAA210872-3) and rabbit anti-MAD2 antibody For blotting immunoprecipitated MAD2, was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human MAD2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAD2 (Cat. No. AAA210872 lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MAD2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MAD2 (Cat. No. AAA210872 lot 4) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-MAD2 antibody
MAD2 (Mitotic Arrest Deficiency 2) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD2 is a subunit of the MCC complex that includes BubR1, Bub3, Cdc20 and Mad2, which may function to inhibit the APC/c (Anaphase Promoting Complex/Cyclosome). MAD2 is regulated by MAD1.
Product Categories/Family for anti-MAD2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,510 Da
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD2A
NCBI Official Synonym Full Names
MAD2 mitotic arrest deficient-like 1 (yeast)
NCBI Official Symbol
MAD2L1
NCBI Official Synonym Symbols
MAD2; HSMAD2
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD2A
UniProt Gene Name
MAD2L1
UniProt Synonym Gene Names
MAD2; HsMAD2
UniProt Entry Name
MD2L1_HUMAN
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Product Notes
The MAD2 mad2l1 (Catalog #AAA210872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MAD2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAD2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the MAD2 mad2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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