Rabbit anti-Human MAD2L1 Polyclonal Antibody | anti-MAD2L1 antibody

Anti-MAD2L1 Antibody, Rabbit Polyclonal

Cat. Num. AAA256096

• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MAD2L1, Antibody; Anti-MAD2L1 Antibody, Rabbit Polyclonal; MAD2L1 Antibody, Rabbit PAb, Antigen Affinity Purified; MAD2 mitotic arrest deficient-like 1 (yeast); Anti-HSMAD2 Antibody; Anti-MAD2 Antibody; anti-MAD2L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MAD2L1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MAD2L1 antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human MAD2L1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MAD2L1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(MAD2L1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-MAD2L1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MAD2L1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size :24 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of MAD2L1 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MAD2L1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human MAD2L1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-MAD2L1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:24 kDaObserved band size:24 kDa)

Related Product Information for anti-MAD2L1 antibody

Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, and MAD2, is a nucleus and cytoplasm protein that belongs to the MAD2 family. MAD2L1 is a component of the spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase-promoting complex by sequestering CDC2 until all chromosomes are aligned at the metaphase plate. MAD2L1 has two highly different native conformations, an inactive open conformation that cannot bind CDC2 and that predominates in cytosolic monomers, and an active closed conformation. MAD2L1 in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed-form and thereby promotes interaction with CDC2.

References

Luo X., et al., 2000, Nat. Struct. Biol. 7:224-229. Sironi L., et al., 2002, EMBO J. 21:2496-2506. Mapelli M., et al., 2007, Cell 131:730-743. Ho C.-Y., et al., 2008, J. Cell. Biochem. 105:835-846. Skinner J.J., et al., 2008, J. Cell Biol. 183:761-768.