IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human MAP2K5 Polyclonal Antibody | anti-MAP2K5 antibody
MAP2K5 Antibody (S149)
Gene Names
MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAP2K5, Antibody; MAP2K5 Antibody (S149); Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5; MAP2K5; MEK5; MKK5; PRKMK5; anti-MAP2K5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAP2K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from human MAP2K5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
127-156
Sequence Length
412
Applicable Applications for anti-MAP2K5 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K5 Antibody (S149) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of MAP2K5 (arrow) using rabbit polyclonal MAP2K5 Antibody (S149). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of MAP2K5 (arrow) using rabbit polyclonal MAP2K5 Antibody (S149). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K5 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-MAP2K5 antibody
MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.
Product Categories/Family for anti-MAP2K5 antibody
References
Raviv,Z.,J. Cell. Sci. 117 (PT 9), 1773-1784 (2004)
Kato,Y.,EMBO J. 16 (23), 7054-7066 (1997)
English,J.M.,J. Biol. Chem. 274 (44), 31588-31592 (1999)
Kato,Y.,EMBO J. 16 (23), 7054-7066 (1997)
English,J.M.,J. Biol. Chem. 274 (44), 31588-31592 (1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50112
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 5 isoform C
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 5
NCBI Official Symbol
MAP2K5
NCBI Official Synonym Symbols
MEK5; MAPKK5; PRKMK5; HsT17454
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 5
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 5
UniProt Gene Name
MAP2K5
UniProt Synonym Gene Names
MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5
UniProt Entry Name
MP2K5_HUMAN
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Product Notes
The MAP2K5 map2k5 (Catalog #AAA285913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 127-156. The MAP2K5 Antibody (S149) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2K5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MAP2K5 map2k5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2K5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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