WB (Western Blot)
(Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).)
Rabbit MAP3K5 Polyclonal Antibody | anti-MAP3K5 antibody
MAP3K5 Antibody(T842)
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human; Predicted Reactivity: Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
MAP3K5, Antibody; MAP3K5 Antibody(T842); Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; MAP3K5; ASK1; MAPKKK5; MEKK5; anti-MAP3K5 antibody
Host
Rabbit
Reactivity
Human; Predicted Reactivity: Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This MAP3K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 821-849 amino acids from human MAP3K5.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
1374
Applicable Applications for anti-MAP3K5 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.
Tissue Location
Abundantly expressed in heart and pancreas.
Antigen Region
821-849 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP3K5 ANtibody (T842), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP3K5 ANtibody (T842), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-MAP3K5 Antibody (T842) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Raji whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 155 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MAP3K5 Antibody (T842) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Raji whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 155 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MAP3K5 antibody
Description: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Product Categories/Family for anti-MAP3K5 antibody
References
Min,W.,Circ. Res. 102 (7), 840-848 (2008). Li,X.,Cell Death Differ. 15 (4), 739-750 (2008). Noguchi,T.,J. Biol. Chem. 283 (12), 7657-7665 (2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
154537 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 5
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol
MAP3K5
NCBI Official Synonym Symbols
ASK1; MEKK5; MAPKKK5
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 5
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 5
UniProt Gene Name
MAP3K5
UniProt Synonym Gene Names
ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5
UniProt Entry Name
M3K5_HUMAN
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Product Notes
The MAP3K5 map3k5 (Catalog #AAA290713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP3K5 Antibody(T842) reacts with Human; Predicted Reactivity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAP3K5 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MAP3K5 map3k5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP3K5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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